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Molecular weight distribution of the aminoacyl-tRNA-synthetases of Escherichia coli by gel filtration

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Summary

Molecular weights of 18 aminoacyl-tRNA-synthetases of E. coli were estimated by gel filtration on Sephadex G-200.

Molecular weights calculated from S20 and D20 values are known for four of the aminoacyl-tRNA-synthetases, the phenylalanyl- (Stulberg, 1967), isoleucyl- (Baldwin and Berg, 1966), tyrosyl- (Calendar and Berg, 1966) and the arginyl-tRNA-synthetase (Stöffler and Nass, 1967). These enzymes have been used as marker proteins for determination of the molecular weights of further 14 aminoacyl-tRNA-synthetases:

  1. 1.

    Nine aminoacyl-tRNA-synthetases have a molecular weight between 90,000 and 120,000, i.e. the asparagyl-, asparaginyl-, glutaminyl-, glycyl-, histidyl-, leucyl-, lysyl-, seryl-and valyl-tRNA-synthetase.

  2. 2.

    Alanyl-tRNA-synthetase: mol. wt. of 180,000.

  3. 3.

    Methionyl- and threonyl-tRNA-synthetase: mol. wt. of 145,000.

  4. 4.

    Prolyl- and glutamyl-tRNA-synthetase: mol. wt. of 75,000.

The reliability of the used gel filtration for molecular weight determination is discussed.

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Authors and Affiliations

  1. Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, Berlin-Dahlem, Germany

    Gisela Nass & Georg Stöffler

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  1. Gisela Nass
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  2. Georg Stöffler
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Nass, G., Stöffler, G. Molecular weight distribution of the aminoacyl-tRNA-synthetases of Escherichia coli by gel filtration. Molec. Gen. Genetics 100, 378–382 (1967). https://doi.org/10.1007/BF00334065

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  • DOI: https://doi.org/10.1007/BF00334065

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