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Ribosomal proteins

II. A rapid micromethod for electrophoretic separation of Escherichia coli ribosomal protein on gelatinized cellulose acetate

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Summary

A new method for electrophoretic separation of ribosomal proteins on gelatinized cellulose acetate is presented. The main features of this method are: 1. fast performance, 2. five to ten micrograms protein are sufficient, 3. the separation of the proteins is mainly due to their net charge, 4. quantitation of separated proteins by densitometry or autoradiography of the original strips is possible. In addition to the described difference in the electrophoretic patterns of E. coli K12 and E. coli B ribosomal proteins in disc polyacrylamide gel electrophoresis (Leboy et al., 1964) some further differences are detected by the CAG electrophoresis.

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Authors and Affiliations

  1. Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, Berlin-Dahlem, Germany

    Georg Stöffler

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  1. Georg Stöffler
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Stöffler, G. Ribosomal proteins. Molec. Gen. Genetics 100, 374–377 (1967). https://doi.org/10.1007/BF00334064

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  • DOI: https://doi.org/10.1007/BF00334064

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