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Transcription of repetitive DNA in Neurospora crassa

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Summary

Repeated DNA sequences of Neurospora crassa were isolated and characterized. Approximately 10–12% of N. crassa DNA sequece were repeated, of which 7.3% were found to be transcribed in mid-log phase of mycelial growth as measured by DNA:RNA hybridization. It is suggested that part of repetitive DNA transcripts in N. crassa were mitochondrial and part were nuclear DNA. Most of the nuclear repeated DNAs, however, code for rRNA and tRNA in N. crassa.

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References

  • Birnstiel, M. L., Chipchase, M., Speirs, J.: “The rRNA cistrons,” Progress in nculeic acids and mol. biol., vol. 11. Davidson and Cohn, editors, p. 352–390. New York: Acad. Press 1971

    Google Scholar 

  • Britten, R. J., Davidson, E. H.: Gene regulation for higher cells: A theory. Science 165, 349–357 (1969)

    Google Scholar 

  • Britten, R. J., Kohne, D. E.: Repeated sequences in DNA. Science 161, 529–540 (1968)

    Google Scholar 

  • Brooks, R. R., Huang, P. C.: Repeated DNA in N. crassa. Biochem. Genet. 6, 41–49 (1972)

    Google Scholar 

  • Brown, R. D., Toechino-Valentini, G. P.: On the role of RNA in gene amplification. Proc. nat. Acad. Sci. (Wash.) 69, 1746 (1972)

    Google Scholar 

  • Chattopadhyay, S. K., Kohne, D. E., Dutta, S. K.: Ribosomal RNA genes of Neurospora: Isolation and characterization. Proc. nat. Acad. Sci. (Wash.) 69, 3256–3259 (1972)

    Google Scholar 

  • Dina, D., Crippa, M., Beccari, E.: Hybridization properties and sequence arrangement in a population of mRNA. Nature (Lond.) New Biol. 242, 101–105 (1973)

    Google Scholar 

  • Dutta, S.K.: Repeated DNA sequences in fungi. Nucleic Acid Res. 1, 1411–1419 (1974)

    Google Scholar 

  • Dutta, S. K.: Transcription of non-repeated DNA in N. crassa. Biochim. biophys Acta (Amst.) 324, 482–487 (1973)

    Google Scholar 

  • Dutta, S. K., Kohne, D. E.: Occurrence of repeated DNA sequences in N. crassa. Proc. Intl. Bot. Congress, Seattle Washington, 50 (1969)

  • Dutta, S. K., Ojha, M.: Relatedness between major taxanomic groups of fungi based on the measurement of DNA nucleotide sequence homology. Molec. gen. Genet. 114, 232–240 (1972)

    Google Scholar 

  • Dutta, S. K., Ray, R.: Partial characterization of tRNA genes of N. crassa. Molec. gen. Genet. 125, 295–300 (1973)

    Google Scholar 

  • Dutta, S. K., Richman, N., Woodward, V. W., Mandel, M.: Measurements of relatedness among broad groups of fungi and plants. Genetics 57, 79–727 (1967)

    Google Scholar 

  • Firtel, R. A.: Changes in the expression of single-copy DNA during development of Dictyostelium discoideum J. molec. Biol. 66, 363–377 (1972)

    Google Scholar 

  • Finkelstein, D. B., Blamire, J., Marmur, J.: Isolation of yeast mitochondrial DNA. Biochem. 11, 4853–4857 (1973)

    Google Scholar 

  • Gelderman, A. H., Rake, A. V., Britten, R. J.: Transcription of nonrepeated DNA in neonatal and fetal mice. Proc. nat. Acad. Sci. (Wash.) 68, 172–176 (1971)

    Google Scholar 

  • Grady, L. J., Campbell, W. P.: Non-repetitive DNA transcription in mouse cells grown in tissue culture. Nature (Lond.) New Biol. 243, 195–198 (1973)

    Google Scholar 

  • Hough, B. R., Davidson, E. H.: Studies of repetitive sequence transcripts of Xenopus oocytes. J. molec. Biol. 70, 491–509 (1972)

    Google Scholar 

  • Kohn, D. E., Byers, M. J.: Amplification and evolution of deoxyribonucleic acid sequences expressed as ribonucleic acid. Biochem. 12, 2373–2378 (1973)

    Google Scholar 

  • Lodish, H. F., Firtel, R. A., Jacobson, A.: Transcription and structure of the genome of the cellular slime mold Dictyostelium discoideum. Cold Spr. Harb. Symp. quant. Biol. 8, 899–914 (1973)

    Google Scholar 

  • Ray, R., Dutta, S. K.: Isolation of tRNA genes from N. crassa. Biochem. biophys. Res. Commun. 47, 1458–1463 (1972)

    Google Scholar 

  • Weiss, H., Jagow, G. von, Klingenberg, M., Bucher, T.: Characterization of N. crassa mitochondria prepared with a grind mill Europ. J. Biochem. 14, 75–82 (1970)

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, Howard University, Washington, D.C.

    S. K. Dutta & R. K. Chaudhuri

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  1. S. K. Dutta
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  2. R. K. Chaudhuri
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Communicated by F. Kaudewitz

This research was supported in part by a contract No. AT(40-1) 4182 with the U.S. Atomic Energy Commission and a grant (GY 10452) from the U.S. National Science Foundation.

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Dutta, S.K., Chaudhuri, R.K. Transcription of repetitive DNA in Neurospora crassa . Molec. gen. Genet. 136, 227–232 (1975). https://doi.org/10.1007/BF00334017

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  • DOI: https://doi.org/10.1007/BF00334017

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