Summary
To define the extent of intervening sequences required for efficient splicing of the CYH2 gene in Saccharomyces cerevisiae, we have constructed a series of intron mutations. Artificial intron extensions of more than 300 bp of the natural intron lead to an inhibition of splicing where-as intron deletions lead to a drastic improvement of the splicing efficiency. It is shown that deletion of a 32 bp sequence element within the intron is responsible for this drastic improvement.
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Communicated by C.P. Hollenberg
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Swida, U., Thüroff, E. & Käufer, N.F. Intron mutations that affect the splicing efficiency of the CYH2 gene of Saccharomyces cerevisiae . Molec Gen Genet 203, 300–304 (1986). https://doi.org/10.1007/BF00333970
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DOI: https://doi.org/10.1007/BF00333970