Summary
We describe the isolation of amethopterin-resistant mutants induced by quinacrine treatment of exponentially growing cultures of Streptococcus pneumoniae. Only mutants located by recombination analysis in a few hundred base pairs were further studied. They were cloned and their DNA sequences show that most of them are ±1-base frame-shift mutants. They are excised and repaired to a degree similar to transition mutants (low efficiency class), suggesting that the mismatches resulting from a transition or a ±1-base mutation are similar substrates for the Hex mismatch repair system.
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Gasc, A.M., Sicard, A.M. Frame-shift mutants induced by quinacrine are recognized by the mismatch repair system in Streptococcus pneumoniae . Molec Gen Genet 203, 269–273 (1986). https://doi.org/10.1007/BF00333965
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DOI: https://doi.org/10.1007/BF00333965