Summary
Strains carrying a dnaA temperature sensitive (t.s.) mutation and a Mu-1 prophage inserted within different genes near the origin of replication have been constructed. For each strain, integratively suppressed Hfrs, named G and D, in which the ori region was replicated clockwise and counterclockwise respectively, were isolated. The strand preferences of Mu-1 specific Okazaki fragments were subsequently determined for each t.s. strain and its Hfr derivatives. Their comparison led us to establish the direction of replication of the Mu-1 marker from ori. The site ori was thus confined to the bglB-C-rbsK-P interval.
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References
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Communicated by W. Arber
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Fayet, O., Louarn, JM. Map position of the replication origin on the E. coli chromosome. Molec. Gen. Genet. 162, 109–111 (1978). https://doi.org/10.1007/BF00333857
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DOI: https://doi.org/10.1007/BF00333857