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New regulatory mutations affecting the expression of the threonine operon in Escherichia coli K-12

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Summary

The promoter of the threonine operon was joined to the structural genes of the lac operon in Escherichia coli K12. The synthesis of β-galactosidase was thus repressed by threonine plus isoleucine in the fusion strains. To isolate mutations which affect the expression of the threonine operon, alterations in the level of expression of the lacZ gene were selected. A new type of regulatory mutation was discovered.

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Authors and Affiliations

  1. Unité de Biochimie Cellulaire, Département de Biochimie et Génétique Microbienne, Institut Pasteur, 25 rue du Dr. Roux, F-75015, Paris, (France)

    Isabelle Saint-Girons

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  1. Isabelle Saint-Girons
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Communicated by G. O'Donovan

This paper is part of a thesis presented by Isabelle Saint-Girons in partial fulfillment of the requirements for the Dr. Sc. degree from the University of Paris

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Saint-Girons, I. New regulatory mutations affecting the expression of the threonine operon in Escherichia coli K-12. Molec. Gen. Genet. 162, 95–100 (1978). https://doi.org/10.1007/BF00333855

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  • DOI: https://doi.org/10.1007/BF00333855

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