Summary
In an E. coli K-12 strain producing a thermolabile, mutagenic DNA polymerase III, enhanced reversion rates were observed for a trp missense mutation of F′trp and ColVBtrp. In addition, replication of these plasmid DNA molecules was greatly reduced at the nonpermissive temperature. Both observations indicate that DNA polymerase III is involved in the replication of these plasmids.
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Communicated by Ch. Auerbach
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Thompson, R., Broda, P. DNA polymerase III and the replication of F and ColVBtrp in Escherichia coli K-12. Molec. gen. Genet. 127, 255–258 (1973). https://doi.org/10.1007/BF00333765
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DOI: https://doi.org/10.1007/BF00333765