Summary
Mutants of Paramecium aurelia resistant to mikamycin were shown by microinjection experiments to be due to changes in the extranuclear, mitochondrial genetic system. These mutants were also resistant to a low concentration of erythromycin.
Double mutants, resistant to a high level of erythromycin and to mikamycin, were obtained by a two-stage mutation-like process. Injection of mitochondria from these resulted in transformation to the double mutant type, irrespective of the selective medium used to obtain the transformants.
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References
Adoutte, A., Beisson, J.: Cytoplasmic inheritance of erythromycin resistant mutations in Paramecium aurelia. Molec. gen. Genet. 108, 80–77 (1970)
Adoutte, A., Beisson, J.: Evolution of mixed populations of genetically different mitochondria in Paramecium aurelia. Nature (Lond.) 235, 393–396 (1972)
Beale, G. H.: A note on the inheritance of erythromycin resistance in Paramecium aurelia. Genet. Res. 14, 341–342 (1969)
Beale, G. H., Knowles, J. K. C., Tait, A.: Mitochondrial genetics in Paramecium. Nature (Lond.) 235, 396–397 (1972)
Bunn, C. L., Mitchell, C. H., Lukins, H. B., Linnane, A. W.: Biogenesis of mitochondria 18. A new class of mitochondrial cytoplasmically determined antibiotic resistant mutants in Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 67, 1233–1244 (1971)
Coen, D., Deutsch, J., Netter, P., Petrochilo, E., Slonimski, P. O.: Mitochondrial genetics I. — Methodology and phenomenology. In: Control of organelle development, (Miller, P. L., ed.) p. 449–496. London: Cambridge University Press 1970
Grivell, L. A., Netter, P., Borst, P., Slominski, P. P.: Mitochondrial antibiotic resistance in yeast: ribosomal mutants resistant to chloramphenicol erythromycin and spiramycin. Biochim. biophys. Acta (Amst.) 312, 358–367 (1973)
Howell, N., Trembath, M. K., Linnane, A. W., Lukins, H. B.: Biogenesis of mitochondria 30. Ananalysis of polarity of mitochondrial gene recombination and transmission. Molec. gen. Genet. (in press) (1973)
Knowles, J. K. C.: Ph. D. Thesis, Edinburgh University (1973)
Knowles, J. K. C.: A study of cytoplasmic erythromycin resistance in Parameciam aurelia using an improved microinjection technique. J. Cell Sci. (in press)
Linnane, A. W., Saunders, G. W., Gingold, E. B., Lukins, H. B.: Biogenesis of mitochondrial 5. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 59, 903–910 (1968)
Sonneborn, T. M.: Methods in the general biology and genetics of Paramecium aurelia. J. exp. Zool. 113, 87–143 (1950)
Tait, A.: Altered mitochondrial ribosomes in an erythromycin resistant mutant of Paramecium. FEBS Letters 24, 117–120 (1972)
Thomas, D. Y., Wilkie, D.: Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance. Genet. Res. 11, 33–41 (1968a)
Thomas, D. Y., Wilkie, D.: Recombination of mitochondrial drug resistance factors in Saccharomyces cerevisiae. Biochem. biophys. Res. Commun. 30, 368–372 (1968b)
Vasquez, D.: Binding of chloramphenicol to ribosomes. Biochim. biophys. Acta (Amst.) 114, 277–288 (1966)
Villa, D., Morimoto, H., Halvorson, H. A.: Mitochondrial and cytoplasmic ribosomal proteins in erythromycin resistant and sensitive yeast strains. Fed. Proc. 31, 456 (abstract) (1972)
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Communicated by G. Magni
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Beale, G.H. Genetic studies on mitochondrially inherited mikamycin-resistance in Paramecium aurelia . Molec. gen. Genet. 127, 241–248 (1973). https://doi.org/10.1007/BF00333763
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DOI: https://doi.org/10.1007/BF00333763