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Régulation de la transcription de l'ADN du bactériophage λ infectant un système membranaire subcellulaire dérivé de sphéroplastes d'Escherichia coli

Regulation of the transcription of bacteriophage λ DNA infecting a membranous system obtained from E. coli spheroplasts

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Summary

In vitro transcription of DNA isolated from mature bacteriophage λ CI857 was studied by infecting a membranous cell-free system obtained from lysates of E. coli spheroplasts. The messenger RNA of lambda was characterized by hybridization with the AT-rich and GC-rich halves of λ DNA molecules and with the two separated strands of the λ genome, as well as by the competition method with λ messenger RNA extracted from induced lysogenic E. coli cells.

The results indicate that the transfer of genetic information by the system is a specifically regulated process leading to formation of “early” and “late” messengers. Transcription is selective and asymmetric.

Chloramphenicol and cytosine arabinoside added at the start of the incubation suppress the synthesis of late mRNA and slightly affect the synthesis of early mRNA.

Synthesis of λ DNA is observed.

DNAase added after 10 minutes of incubation, does not prevent an important synthesis of late mRNA.

Synthesis of λ DNA and proteins appears to be a prerequisite for the transcription of late cistrons.

The prominent role of a complex between λ DNA and membranes in the production of mRNA and particularly in the selective and asymmetric transcription of λ DNA into late messenger is emphasized.

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  1. Laboratoire d'Enzymologie microbienne Equipe de Recherches No 21, C.N.R.S., Gif-sur-Yvette, France

    Alain Lévine, Huguette J. Lévine & Bention Nisman

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  1. Alain Lévine
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  2. Huguette J. Lévine
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  3. Bention Nisman
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Lévine, A., Lévine, H.J. & Nisman, B. Régulation de la transcription de l'ADN du bactériophage λ infectant un système membranaire subcellulaire dérivé de sphéroplastes d'Escherichia coli . Molec. Gen. Genetics 107, 173–193 (1970). https://doi.org/10.1007/BF00333633

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