Summary
Two closely linked mutations, U20 and M87, in the deletion group V. of the transferase gene of the galactose operon in E. coli were crossed against a set of other mutations in the epimerase, transferase and kinase genes of the galactose operon by P1 transduction. U20 is an amber mutation. Its Gal + frequencies are higher by a factor of 2 to 10 than those of M87. Two double mutants of U20 with other gal mutations do not show these Gal + frequencies in similar crosses. The results can be explained on the assumption that in heteroduplexes U20 is repaired to yield wildtype at higher rate than is M87.
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Communicated by F. Stahl
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Kemper, B. Increased recombinant frequencies with an Amber mutation in the Gal-operon of E. coli . Molec. Gen. Genetics 107, 107–113 (1970). https://doi.org/10.1007/BF00333626
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DOI: https://doi.org/10.1007/BF00333626