Summary
The UV enhancement of recombination among λ bacteriophages was studied in host bacteria that are defective in DNA repair and compared to that observed in wild type host strains. The following conclusions were drawn:
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1.
The UV enhancement of recombination is even greater in HCR− than in HCR+ host strains. The UV lesions that promote recombination are repaired by host cell reactivation with the same efficiency as are potentially lethal UV lesions.
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2.
The UV enhancement of λ recombination is the same in HCR+ host strains that are deficient in their own recombination as it is in the wild type hosts.
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3.
Very few lesions per phage particle are sufficient to stimulate the enhanced recombination. The UV-stimulated recombination occurs during the same time interval as host cell reactivation.
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4.
There is substantial multiplicity reactivation of UV lesions in λ. Similar numbers of recombination events neutralize similar numbers of UV lesions in HCR+ and HCR− hosts, but a much greater survival increase is observed in the latter.
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Baker, R.M., Haynes, R.H. UV-Induced enhancement of recombination among lambda bacteriophages in UV-sensitive host bacteria. Molec. Gen. Genetics 100, 166–177 (1967). https://doi.org/10.1007/BF00333603
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DOI: https://doi.org/10.1007/BF00333603