Summary
We have examined the 5′ flanking region of a Drosophila melanogaster tRNA Val4 gene for DNA sequences that influence in vitro transcription efficiency in a homologous, cell-free transcription reaction. Comparison of the template efficiencies of a set of deletion mutants indicated both inhibitory and stimulatory sequences in the 5′ flanking region. When sequences between positions-49 and-70, relative to the start of the coding region of the mature tRNA gene, were deleted template activity increased. Deletions to-10 resulted in a 100-fold drop in the rate of transcription directed by the template with the primary effect resulting from deletion of the nucleotides between-38 and-34. The nucleotides between-38 and-34 may be an example of one general type of positive transcription modulator.
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Communicated by E. Bautz
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Sajjadi, F.G., Miller, R.C. & Spiegelman, G.B. Identification of sequences in the 5′ flanking region of a Drosophila melanogaster tRNA Val4 gene that modulate its transcription in vitro. Mol Gen Genet 206, 279–284 (1987). https://doi.org/10.1007/BF00333585
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DOI: https://doi.org/10.1007/BF00333585