Summary
In this paper we report the precise excision of the group I intron aI2b from the cox1 gene and of the group II intron bI from the cob gene fo the Schizosaccharomyces pombe strain 50. We present evidence that DNA excision of both intron DNA sequences is under nuclear control. Attempts to remove the first cox1 intron (aI1) have failed so far, but a deletion of approximately 200 bp in the open intronic reading frame demonstrates that it is not essential for normal cellular functions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- cox1, cox2, cox3 :
-
genes encoding subunits 1, 2 and 3 of cytochrome c oxidase
- cob :
-
gene encoding apocytochrome b
- rns and rnl :
-
genes encoding the small and large ribosomal RNA
- atp6, atp8 and atp9 :
-
genes encoding subunits 6, 8, and 9 of the ATP synthase complex
- urfa :
-
unassigned reading frame a
- aI1, aI2a, aI2b, aI3 :
-
introns in the cox 1 gene of S. pombe
- bI :
-
intron in the cob gene
- del-aI2b and del-bI :
-
respiratory competent strains in which the respective introns have been deleted by DNA splicing
References
Ahne F, Merlos-Lange AM Lang BF, Wolf K (1984) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe. 5. Characterization of mitochondrial deletion mutants. Curr Cenet 8:517–524
Alwine JC, Kempe DJ, Parker BA, Reiser J, Renart J, Stark GR, Wahl GM (1979) Defection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol 68:220–242
Been MD, Cech TR (1985) Sites of circularization of the Tetrahymena rRNA IVS are determined by sequence and influenced by position and secondary structure. Nucleic Acids Res 13:8389–8407
Carignani G, Netter P, Bergantino E, Robineau S (1986) Expression of the mitochondrial split gene coding for cytochrome oxidase subunit I in S. cerevisiae: RNA splicing pathway. Curr Genet 11:55–64
Clewell DB (1972) Nature of ColEI plasmid replication in E. coli in the presence of chloramphenicol. J Bacteriol 110:667–676
Davies RW, Waring RB, Ray JA, Brown TA, Scazzocchio C (1982) Making ends meet: a model for RNA splicing in fungal mitochondria. Nature 300:719–724
Flavell A (1985) Introns continue to amaze Nature 316:574–575
Gargouri A, Lazowska J, Slonimski PP (1983) DNA-splicing of introns in a gene: a general way of reverting intron mutations. in: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983, Walter de Gruyter, Berlin New York, pp 259–268
Kotylak Z, Lazowska J, Slonimski PP (1985) Intron encoded proteins of mitochondria: Key elements of gene expression and genomic evolution. In: Quagliariello E, Slater EC, Palmieri F, Saccone C, Kroon AM (eds) Achievements and perspectives of mitochondrial research. Vol II: Biogenesis. Elsevier Science Publishers, Amsterdam New York Oxford, pp 1–20
Kück U, Osiewacz HD, Schmidt U, Kappelhoff B, Schulte E, Stahl U, Esser K (1985) The onset of senescence is affected by DNA rearrangements of a discontinuous mitochondrial gene in Podospora anserina. Curr Genet 9:373–382
Lang BF (1984) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe: highly homologous introns are inserted at the same position of the otherwise less conserved cox1 genes in Schizosaccharomyces pombe and Aspergillus nidulans. EMBO J 3:2129–2136
Lang BF, Wolf K (1984) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe. 2. Localization of genes by interspecific hybridization in strain ade7-50h - and cloning of the genome in small fragments. Mol Gen Genet 196:465–472
Lang BF, Ahne F, Distler S, Trinkl H, Kaudewitz F, Wolf K (1983) Sequence of the mitochondrial DNA, arrangement of genes and processing of their transcripts in Schizosaccharomyces pombe. In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983, Walter de Gruyter, Berlin New York, pp 313–329
Lang BF, Ahne F, Bonen L (1985) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe: The cytochrome b gene has an intron closely related to the first two introns in the Saccharomyces cerevisiae cox1 gene. J Mol Biol 184:353–366
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78
Messing J, Vieira J (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276
Michel F, Dujon B (1983) Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast-and nuclear-encoded members. EMBO J 2:33–38
Michel F, Lang BF (1985) Mitochondrial class II introns encode proteins related to the reverse transcriptase of retroviruses. Nature 316:641–643
Perea J, Jacq C (1985) Role of the 5′ haipin structure in the splicing accuracy of the fourth intron of the yeast cob-box gene. EMBO J 4:3281–3288
Rigby PW, Dieckman M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Seitz-Mayr G, Wolf K (1982) Extrachromosomal mutator inducing point mutations and deletions in the mitochondrial genome of fission yeast. Proc Natl Acad Sci USA 79:2618–2622
Southern EM (1975) Detection of specific sequences among DNA fragments by gel electrophoresis. J Mol Biol 98:503–517
Steinhilber W, Cummings DJ (1986) A DNA polymerase activity with characteristics of a reverse transcriptase in Podospora anserina. Curr Genet 10:389–392
Trinkl H, Wolf K (1986) The mosaic cox1 gene in the mitochondrial genome of Schizosaccharomyces pombe: minimal structural requirements and evolution of group I introns. Gene 45:289–297
Trinkl H, Lang BF, Wolf K (1985) The mitochondrial genome of the fission yeast Schizosaccharomyces pombe. 7. Continuous gene for apocytochrome b in strain EF1 (CBS 356) and sequence variation in the region of intron insertion in strain ade7-50h -. Mol Gen Genet 198:360–363
Waring RB, Davies RW (1984) Assessment of a model of intron RNA secondary structure relevant to RNA self-splicing — a review. Gene 28:277–291
Waring RB, Ray JA, Edwards SW, Scazzocchio C, Davies RW (1985) The Tetrahymena rRNA intron self-splices in E. coli: in vivo evidence for the importance of key base-paired regions of RNA for RNA enzyme function. Cell 40:371–390
Author information
Authors and Affiliations
Additional information
Communicated by W. Gajewski
Rights and permissions
About this article
Cite this article
Merlos-Lange, A.M., Kanbay, F., Zimmer, M. et al. DNA splicing of mitochondrial group I and II introns in Schizosaccharomyces pombe . Mol Gen Genet 206, 273–278 (1987). https://doi.org/10.1007/BF00333584
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00333584