Summary
The α-methylglucoside fermenting enzymes produced in response to the two complementing gene pairs (MGL1 MGL2) and (MGL3 MGL2) were purified and certain of their physical properties were compared. Although both enzymes have the same molecular weight, substrate and serological specificities, they differ with respect to their specific activity, Michaelis constant and heat stability. On the basis of these findings we argue that the loci MGL1 and MGL3 are the structural genes responsible for the production of the α-methylglucosidases in yeast and that the loci controlling maltase and α-methylglucosidase production arose from a common ancestor by gene duplication.
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Communicated by F. Kaudewitz
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Khan, N.A., Haynes, R.H. Genetic redundancy in yeast: Non-identical products in a polymeric gene system. Molec. gen. Genet. 118, 279–285 (1972). https://doi.org/10.1007/BF00333464
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DOI: https://doi.org/10.1007/BF00333464