Summary
No suppressible alleles have been found at the his1 locus of Saccharomyces cerevisiae. Seventeen noncomplementing alleles have been tested against the strong ochre suppressor SUP2. These alleles, plus an additional 34 complementing alleles, were previously tested against a weaker suppressor SUP11. These results, which are in marked contrast to experience with other yeast loci where suppressible alleles are frequent, may be explained if the his1 product is transcribed from a polycistronic message so that nonsense mutations lead to loss of a second nonsupplementable function, or if the his1 protein serves as an essential subunit for an unknown enzyme complex.
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Communicated by F. Kaudewitz
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Snow, R. Absence of suppressible alleles at the his 1 locus of yeast. Molec. Gen. Genet. 164, 341–342 (1978). https://doi.org/10.1007/BF00333166
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DOI: https://doi.org/10.1007/BF00333166