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Bacillus subtilis dnaF: A mutation of the gene specifying the structure of DNA polymerase III

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Summary

The characteristics of Bacillus subtilis dnaF, a mutation specifying a temperature sensitive phenotype, were examined to determine its relationship to polC, the gene specifying the structure of DNA polymerase III (pol III). Exposure of growing cells bearing dnaF to non-permissive temperature inhibited replicative DNA synthesis and specifically depressed the expression of pol III activity in crude extracts. Highly purified pol III derived from cells bearing dnaF was temperature sensitive in its polymerase activity, indicating that dnaF is a specific, polC mutation which specifies a structurally altered enzyme.

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References

  • Anagnostopoulos, G., Spizizen, J.: Requirements for transformation in B. subtilis. J. Bact. 81, 741–746 (1961)

    Google Scholar 

  • Attolini, C., Mazza, G., Fortunato, A., Garrocchi Mastromei, G., Riva, S., Falaschi, A.: On the identity of dnaP and dnaF genes of Bacillus subtilis. Molec. gen. Genet. 148, 9–17 (1976)

    Google Scholar 

  • Bazill, G.W., Gross, J.d.: Mutagenic DNA polymerase in B. subtilis. Nature (Lond.) New Biol. 243, 241–243 (1973)

    Google Scholar 

  • Brown, N.C.: Inhibition of bacterial DNA replication by 6-(p-Hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis. J. molec. Biol. 59, 1–17 (1971)

    Google Scholar 

  • Brown, N.C., Gambino, J., Wright, G.E.: Inhibitors of Bacillus subtilis DNA polymerase III. 6-(arylalkylamino) uracils and 6-anilinouracils. J. med. Chem. 20, 1186–1189 (1977)

    Google Scholar 

  • Burton, K.: A Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315–323 (1956)

    Google Scholar 

  • Clements, J., D'Ambrosio, J., Brown, N.: Inhibition of Bacillus subtilis deoxyribonucleic acid polymerase III by phenylhydrazinopyrimidines. Demonstration of a drug-induced deoxyribonucleic acid-enzyme complex. J. biol. Chem. 250, 522–526 (1975)

    Google Scholar 

  • Fujimura, R.: Nucleotide analysis of deoxyribonucleic acid containing deoxybromouridylic acid. Analyt. Biochem. 36, 62–71 (1970)

    Google Scholar 

  • Gefter, M.L., Hirota, Y., Kornberg, T., Wechsler, J., Barnoux, C.: Analysis of DNA polymerases II and III in mutants of Escherichia coli thermosensitive, for DNA synthesis. Proc. nat. Acad. Sci. (Wash.) 72, 3150–3153 (1971)

    Google Scholar 

  • Harris-Warwick, R.M., Elkana, Y., Ehrlich, S.D., Lederberg, J.: Electrophoretic separation of Bacillus subtilis genes. Proc. nat. Acad. Sci. (Wash.) 72, 2207–2211 (1975)

    Google Scholar 

  • Karamata, D., Gross, J.D.: Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Molec. gen. Genet. 108, 277–287 (1970)

    Google Scholar 

  • Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227, 680–685 (1970)

    Google Scholar 

  • Love, E., D'Ambrosio, J., Brown, N., Dubnau, D.: Mapping of the gene specifying DNA polymerase III of Bacillus subtilis. Molec. gen. Genet. 144, 313–321 (1976)

    Google Scholar 

  • Low, R., Rashbaum, S., Cozzarelli, N.: Purification and characterization of DNA polymerase III from Bacillus subtilis. J. biol. Chem. 251, 1311–1325 (1976)

    Google Scholar 

  • Lowry, O.H., Rosebourgh, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    Google Scholar 

  • Mackenzie, J.M., Neville, M.N., Wright, G.E., Brown, N.C.: Hydroxyphenylazopyrimidines. Characterization of the active forms and their inhibitory action on a DNA polymerase from Bacillus subtilis. Proc. nat. Acad. Sci. (Wash.) 70, 512–516 (1973)

    Google Scholar 

  • Neville, M.N., Brown, N.C.: Inhibition of a discrete bacterial DNA polymerase by 6-(p-hydroxyphenylazo)-uracil and 6-(p-hydroxyphenylazo)-isocytosine. Nature (Lond.) NewBiol. 240, 80–88 (1972)

    Google Scholar 

  • Riva, S., Van Sluis, C., Mastromei, G., Attolini, C., Mazza, G., Polsinelli, M., Falaschi, A.: A new mutant of Bacillus subtilis altered in the initiation of chromosomal replication. Molec. gen. Genet. 137, 185–202 (1975)

    Google Scholar 

  • Vrooman, M.J., Brown, N.: Bacillus subtilis dnaF: a conditional polC mutation affecting the exonuclease activity of DNA polymerase III. Fed. Proc. 36, 849 (1977)

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, University of Massachusetts Medical School, 01605, Worcester, MA, USA

    Mary Jane Vrooman, Marjorie H. Barnes & Neal C. Brown

Authors
  1. Mary Jane Vrooman
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  2. Marjorie H. Barnes
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  3. Neal C. Brown
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Communicated by G. O'Donovan

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Vrooman, M.J., Barnes, M.H. & Brown, N.C. Bacillus subtilis dnaF: A mutation of the gene specifying the structure of DNA polymerase III. Molec. Gen. Genet. 164, 335–339 (1978). https://doi.org/10.1007/BF00333165

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  • DOI: https://doi.org/10.1007/BF00333165

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