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Extrachromosomal inheritance in Schizosaccharomyces pombe

VII. Studies by zygote clone analysis on transmission, segregation, recombination, and uniparental inheritance of mitochondrial markers conferring resistance to antimycin, chloramphenicol, and erythromycin

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Summary

Crosses involving mitochondrial markers conferring resistance to antimycin (anar, AR), chloramphenicol (capr, CR), and erythromycin (eryr, ER) in cis- and trans-configuration were studied by zygote clone analysis. Mutant ana r-8 from which all other drug-resistant isolates were derived, exhibits a highly biased transmission (6.8% ana r), in an analysis of 100 individual zygote clones. Important results of zygote clone analyses were:

  • - Zygote clones may contain one, two, three, or four mitochondrial genotypes.

  • - The proportion of the two parental and the two recombinant genotypes in individual zygote clones can vary almost over the entire range of percentages.

  • - Proportions of the two corresponding recombinant types in individual clones are usually unequal.

  • - Transmission rates of markers are higher in trans-than in cis-crosses indicating additivity of bias by two mutated alleles in coupling.

  • - Transmission rates are different for the three markers both in cis- and trans-crosses, being lowest for C Rand highest for E R.

  • - Up to more than 80% uniform clones, expressing only one genotype, can be produced in cis- and trans-crosses. In cis-crosses always the double-sensitive parental type becomes uniform, in trans-crosses this may be the case for parental and/or recombinant genotypes.

A tentative map is presented using data from cis- and trans-crosses, including a correction by omission of uniform clones. Phenomena of transmission, segregation, and formation of uniform clones are discussed with special regard to the difference brought about by fission versus budding.

A comparison with relevant data from Saccharomyces cerevisiae and other organisms is presented.

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Authors and Affiliations

  1. Genetisches Institut der Universität München, Maria-Ward-Str. 1a, D-8000, München 19, Federal Republic of Germany

    G. Seitz-Mayr, K. Wolf & F. Kaudewitz

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  1. G. Seitz-Mayr
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  2. K. Wolf
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  3. F. Kaudewitz
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Communicated by W. Gajewski

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Seitz-Mayr, G., Wolf, K. & Kaudewitz, F. Extrachromosomal inheritance in Schizosaccharomyces pombe . Molec. Gen. Genet. 164, 309–320 (1978). https://doi.org/10.1007/BF00333162

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  • DOI: https://doi.org/10.1007/BF00333162

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