Summary
Invertase formation in the yeast Saccharomyces cerevisiae is subject to repression by hexoses in the growth medium. Mutagen-induced (ethyl methanesulfonate or N-methyl-N'-nitro-nitrosoguanidine) invertase hyperproducer mutants have been derived from the SUC3 MAL3 strain EK-6B by selecting for their ability to grow on media containing the sugar raffinose plus 2-deoxy-D-glucose (2DG). Raffinose like sucrose is a β-fructoside which can be hydrolyzed by yeast invertase (β-fructofuranoside fructohydrolase). These mutants, designated dgr, produce higher levels of invertase (α-glucosidase levels are also elevated but to a lesser extent) under conditions normally repressing invertase biosynthesis in the parent. Invertases of mutants dgr2 and dgr3 are indistinguishable from that of EK-6B with respect to their Km's for sucrose and thermal labilities. Genetic studies revealed that dgr2 and dgr3 are recessive and unlinked to the SUC3 gene.
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Communicated by F. Kaudewitz
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Hackel, R.A., Khan, N.A. Genetic control of invertase formation in Saccharomyces cerevisiae . Molec. Gen. Genet. 164, 295–302 (1978). https://doi.org/10.1007/BF00333160
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DOI: https://doi.org/10.1007/BF00333160