Skip to main content
SpringerLink
Log in

A DNA segment within the colicin E1 structural gene on ColE1 affecting immunity to colicin

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Insertion of DNA at the EcoRI site of ColE1 results in increase of immunity to colicin killing in E. coli harboring such recombinant ColE1 plasmid as compared to E. coli (ColE1). This effect is neither due to cis or trans interactions originating from the inserted foreign DNA fragment, nor to changes in plasmid copy number. This defect in the immunity mechanism is not trans complemented for by wild type ColE1. Increase in immunity can also be obtained by deleting a DNA segment from the ColE1 genome. This segment is ∼120 bp left to the EcoRI site within the colicin structural gene. It is concluded that the structure of DNA per se, around the EcoRI site, within colicin structural gene, is the structure which affects immunity expression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bertrand, K., Korn, L., Lee, F., Platt, T., Squires, C.L., Squires, C., Yanofsky, C.: New features of the regulation of the tryptophane operon. Science 189, 22–26 (1975)

    Google Scholar 

  • Clewell, D.B., Helinski, D.: Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 DNA and protein in E. coli. Biochemistry 9, 4428–4440 (1970)

    Google Scholar 

  • Cohen, S.N., Chang, A.C.Y., Boyer, H.W., Helling R.B.: Construction of biologically functional bacterial plasmid in vitro. Proc. natn. Acad. Sci. U.S.A. 70, 3240–3244 (1973)

    Google Scholar 

  • Dougan, G., Sherratt, D.J.: The transposon Tnl as a probe for studying ColE1 structure and function. Molec. Gen. Genet. 151, 151–160 (1977)

    Google Scholar 

  • Fredericq, P.: Colicins and colicinogenic factors. Symp. Soc. Exptl. Biol. 12, 104–122 (1958)

    Google Scholar 

  • Gottesman, S., Adhya, S.: In: DNA insertion elements, plasmids and episomes (Eds. A.L. Bukhari, J.A. Shaprio and S.L. Adhya), pp. 713. New York: Cold Spring Harbor Laboratory 1977

    Google Scholar 

  • Green, P.J., Betlach, M.C., Boyer, H.W.: In: Methods in molecular biology (R.B. Wickner, ed.) 7, 87–111 (1974)

  • Hershfield, V., Boyer, H.W., Yanofsky, C., Lovett, M.D., Helinski, D.R.: Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA. Proc. natn. Acad. Sci. USA 71, 3455–3459 (1974)

    Google Scholar 

  • Inselburg, J.: Isolation, mapping and examination of effects of TnA insertions in ColE1 plasmids. J. Bacteriol. 129, 482–491 (1976)

    Google Scholar 

  • Inselburg, J.: Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid. J. Bacteriol. 132, 332–340 (1977)

    Google Scholar 

  • Jakes, K., Zinder, N.D., Boon, T.: Purification, and properties of colicin E3 immunity protein. J. Biol. Chem. 249, 438–444 (1974)

    Google Scholar 

  • Levisohn, R., Konisky, J. and Nomura, M.: Interaction of colicins with bacterial cells. J. Bacteriol. 96, 811–821 (1968)

    Google Scholar 

  • Mandel, M., Higa, A.: Calcium dependent bacteriophage DNA infection. J. molec. Biol. 53, 159–162 (1970)

    Google Scholar 

  • Meagher, R.B., Tait, R.C., Betlach, M., Boyer, H.W.: Protein expression in E. coli minicells by recombinant plasmids. Cell 10, 521–536 (1977)

    Google Scholar 

  • Ohmori, H., Tomizawa, J.I.: In: DNA insertion elements, plasmids and episomes (Eds. A.I. Bukhari, J.A. Shapiro and S.L. Adhya) pp. 682. New York: Cold Spring Harbor Laboratory 1977

    Google Scholar 

  • Shafferman, A., Cohen, S.: Tetracycline resistance expressed by pSC101 hybrids. In: Extrachromosomal inheritance in bacteria-Oholo (eds. Hertman, I., Shafferman, A. and Cohen, A.) Basel: Karger (in press) 1978

    Google Scholar 

  • Sheratt, D.J., Dougan, G., Saul, M., Sunar, B., Twigg, A., Warren, G.: Structure and function of plasmid ColE1 and related Plasmids. In: Extrachromosomal inheritance in bacteria-Oholo (Eds. Hertman, I., Shafferman, A. and Cohen, A.), Basel: Karger in press 1978

    Google Scholar 

  • Sidikaro, J., Nomura, M.: E3 immunity substance. J. Biol. Chem. 249, 445–453 (1974)

    Google Scholar 

  • Tanaka, T., Weisblum, B.: Construction of colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J. Bacteriol. 121, 354–362 (1975)

    Google Scholar 

  • Weiss, B., Jacquemin-Sablon, A., Live, R.T., Fareed, G.C., Richardson, C.C.: Enzymatic breakage and joining of deoxyribonucleic acid. J. Biol. Chem. 243, 4543–4555 (1968)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Israel Institute for Biological Research, Ness-Ziona, Israel

    A. Shafferman, S. Cohen & Y. Flashner

Authors
  1. A. Shafferman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Cohen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Flashner
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by E. Bautz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shafferman, A., Cohen, S. & Flashner, Y. A DNA segment within the colicin E1 structural gene on ColE1 affecting immunity to colicin. Molec. Gen. Genet. 164, 259–264 (1978). https://doi.org/10.1007/BF00333155

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00333155

Keywords

Search

Navigation