Biochemical and genetic studies of recombination proficiency in Escherichia coli K12
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Residual genetic recombination is carried out by recB-recC- mutants of E. coli. Recombinants (for one gene) formed by a recB-recC- parent were shown to be as recombination deficient as their parent, when recombination of a second gene is measured. Therefore the resididual recombination cannot be attributed to a genetically recombination proficient fraction of the parent recB-recC- culture. I conclude that each recB-recC- parent cell is capable of carrying out genetic recombination. This conclusion is consistent with the existence of an alternate (and minor) recombination mechanism in E. coli K12, independent of the recB+recC+ mediated steps.
KeywordsRecombination Genetic Study Parent Cell Genetic Recombination Recombination Mechanism
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