Summary
Recombination between the tandem duplicated segments of λb221a106-15 yields unduplicated (“single-copy”) λb221 phage. The apparent frequency of intramolecular events among these recombinations was determined for both cellular (“Rec”) and bacteriophage (“Red”) generalized recombination systems. The progeny from single-cycle growth experiments with genetically marked duplication phages were treated with EDTA to inactivate all but the singlecopy phages produced by recombination. Analysis of the genotypes of the EDTA-resistant phages suggested that intramolecular events were about 1 to 5 times as frequent as intermolecular ones. While the results suggest that intramolecular events are not intrinsically forbidden, the quantitative values for the ratio depend on the assumption that intracellular phage chromosomes are completely mixed.
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Communicated by B.A. Bridges
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Hays, J.B., Zagursky, R.J. Generalized recombination in tandem duplications of bacteriophage lambda. Molec. Gen. Genet. 160, 325–330 (1978). https://doi.org/10.1007/BF00332976
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DOI: https://doi.org/10.1007/BF00332976