Summary
The host-controlled K restriction of unmodified phage λ was 10-100-fold alleviated in the wild-type strain E. coli K12, carrying plasmid pKM101 of incompability group N. pKM101-mediated release of K restriction was also observed in lexA -, recA -, and recB - strains of E. coli K12. By restriction mapping Tn5 insertions in pKM101, which reduced pKM101-mediated alleviation of restriction, were shown to be located within the BglIIB fragment approximately 11 kb anticlockwise from the RI site of pKM101. We have termed the gene(s) promoting the alleviation of K restriction of phage λ ard (alleviation of restriction of DNA). It was shown (1) that ard function affected only the EcoK restriction system and not the EcoB, EcoRI, EcoRIII, or EcoPI system, (2) ard gene(s) did not mediate EcoK type modification of λ DNA and did not increase the modification activity of the EcoK system in a way similar to that observed with gene ral of bacteriophage λ.
Similar content being viewed by others
References
Arber W (1974) DNA modification and restriction. Prog Nuceic Acid Res Mol Biol 14:1–37
Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev 44:1–56
Belogurov AA, Zavilgelsky GB (1983) Effect of plasmid pKM101 on K-specific restriction and modification of DNA of bacteriophage lambda. Proceedings of USSR Academy of Sciences 269:738–741
Berg DE (1977) Insertion and excision of the transposable kanamycin resistance determinant Tn5. In: Buchari AJ, Shapiro JA, Adhya SL (eds) DNA insertion elements plasmids and episomes. Cold Spring Harobr Laboratory. Cold Spring Harbor, New York
Bickle TA (1982) The ATP-dependent restriction endinucleases. In: Linn S, Roberts R (eds) Nucleases. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 85–108
Boyer HW (1971) DNA restriction and modification mechanism in bacteria. Annu Rev Microbiol. 25:135–176
Day RS (1977) UV-induced alleviation of K-specific restriction of bacteriophage λ. J Virol 21:1249–1251
Krüger DH, Schroeder C (1981) Bacteriophage T3 and bacteriophage T7 virus-host cell interactions. Microbiol Rev 45:9–51
Langer PJ, Shanabruch WG, Walker GC (1981) Functional organisation of plasmid pKM101. J Bacteriol 145:1310–1316
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 92–93
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 187
Shanabruch WG, Walker GC (1980) Localization of the plasmid (pKM101) gene(s) involved in recA+lexA+ dependent mutagenesis. Mol Gen Genet 179:289–297
Studier FW (1975) Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J Mol Biol 94:283–295
Thoms B, Wackernagel W (1982) UV-induced alleviation of restriction in E. coli K-12: Kinetics and induction and specificity of the SOS function. Mol Gen Genet 186:111–117
Toothman P (1981) Restriction alleviation by bacteriophage lambda and lambda reverse. J Virol 38:621–631
Yuan K (1981) Structure and mechanism of multifunctional restriction endonucleases. Ann Rev Biochem 50:85–102
Zabeau M, Friedman S, Van Montagu M, Schell J (1980) The ral gene of phage λ 1. Identification of non-essential gene that modulates restriction and modification in E. coli. Mol Gen Genet 179:63–74
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Belogurov, A.A., Yussifov, T.N., Kotova, V.U. et al. The novel gene(s) ARD of plasmid pKM101: alleviation of EcoK restriction. Mol Gen Genet 198, 509–513 (1985). https://doi.org/10.1007/BF00332948
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332948