Skip to main content
SpringerLink
Log in

A change of threonine 266 to isoleucine in the lac permease of Escherichia coli diminishes the transport of lactose and increases the transport of maltose

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The DNA sequence of several functionally interesting lac permease mutants of Escherichia coli has been determined. The phenotypes of the mutant permeases were described by Mieschendahl et al. (1981). The following exchanges are noteworthy: tyr to asp in codon 26 in Y -K MUB 7; thr to ile in codon 266 in Y -K AJ 33; gly to asp in codon 262 in Y -D 3 and in Y -D 4.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bocklage H (1982) Klonierung und Sequenzierung von Mutanten im lac Y Gen von E. coli. Diplomarbeit, Köln

  • Bocklage H, Müller-Hill B (1983) Lac Z - Y + fusions in Escherichia coli. Eur J Biochem 137:561–565

    Google Scholar 

  • Büchel D, Gronenborn B, Müller-Hill B (1980) Sequence of the lactose permease gene. Nature 283:541–545

    Google Scholar 

  • Clewell D, Helinski P (1969) Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc Natl Acad Sci USA 62:1159–1166

    Google Scholar 

  • Ehring R, Beyreuther KT, Wright JK, Overath P (1980) In vitro and in vivo products of E. coli lactose permease gene are identical. Nature 283:537–540

    Google Scholar 

  • Goldkorn T, Rimon G, Kempmerl ES, Kaback HR (1984) Functional molecular weight of the lac carrier protein from Escherichia coli as studied by radiation inactivation analysis. Proc Natl Acad Sci USA 81:1021–1025

    Google Scholar 

  • Hobson AC, Gho D, Müller-Hill B (1977) Isolation, genetic analysis and characterisation of Escherichia coli mutants with defects in the lacY gene. J Bacteriol 131:830–833

    Google Scholar 

  • Kaback HR (1983) The lac carrier protein of Escherichia coli. J Membr Biol 76:95–112

    Google Scholar 

  • Klenow H, Henningsen J (1970) Selective elimination of the exonuclease activity of the desoxyribonucleic acid polymerase of Escherichia coli B by limited proteolysis. Proc Natl Acad Sci USA 65:168–175

    Google Scholar 

  • Koenen M (1984) Klonierung von Genfragmenten und ihre Identifizierung über die antigenen Eigenschaften ihrer Genprodukte. Dissertation, Köln

  • Marnius MG, Morris NR (1973) Isolation of desoxyribonucleic acid methylase mutants of Escherichia coli K 12. J Bacteriol 114:1143–1150

    Google Scholar 

  • Markgraf M (1984) Analyse des Y-Gens aus dem lac-Operon von E. coli. Diplomarbeit, Köln

  • Maxam A, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74:560–564

    Google Scholar 

  • Mieschendahl M, Büchel D, Bocklage H, Müller-Hill B (1981) Mutations in the lacY gene of Escherichia coli define functional organisation of lactose permease. Proc Natl Acad Sci USA 78:7652–7656

    Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Overath P, Wright JK (1983) Lactose permease: a carrier on the move. TIBS 8:404–408

    Google Scholar 

  • Rüther U (1982) pUR 250 allowes rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Res 10:5764–5772

    Google Scholar 

  • Rüther U (1983) Methoden zur Klonierung, Sequenzierung und Identifizierung von DNA-Sequenzen. Dissertation, Köln

  • Shuman HA, Beckwith J (1979) Escherichia coli K12 mutants that allow transport of maltose via the β-galactoside transport system. J Bacteriol 137:365–373

    Google Scholar 

  • West IC, Mitchell P (1973) Stoicheiometry of lactose-protein symport across the plasma membrane of Escherichia coli. Biochem J 132:587–592

    Google Scholar 

  • Wilson TH, Seto-Young D, Bedu S, Putzrath R, Müller-Hill B (1984) A study of mutants of the lactose transport system of E. coli. In: Adelberg EA, Slayenen CW (eds) Current Topics in Membranes and Transport XXIII, “Gene and Membranes”. Academic Press, San Diego, in press

    Google Scholar 

  • Wright JK, Weigel U, Lustig A, Bocklage H, Mieschendahl M, Müller-Hill B, Overath P (1983) Does the lactose carrier of Escherichia coli function as a monomer? FEBS Lett 162:11–15

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Genetik, Universität Köln, D-5000, Köln 41, Federal Republic of Germany

    Martina Markgraf, Hubertus Bocklage & Benno Müller-Hill

Authors
  1. Martina Markgraf
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hubertus Bocklage
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Benno Müller-Hill
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gehring

Rights and permissions

Reprints and permissions

About this article

Cite this article

Markgraf, M., Bocklage, H. & Müller-Hill, B. A change of threonine 266 to isoleucine in the lac permease of Escherichia coli diminishes the transport of lactose and increases the transport of maltose. Mol Gen Genet 198, 473–475 (1985). https://doi.org/10.1007/BF00332941

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00332941

Keywords

Search

Navigation