Summary
Plasmid pC194-1, a mutant of pC194, and chimeric derivatives of pC194-1 are segregationally unstable in B. subtilis. Such instability could be enhanced by exposure of pC194-1-carrying cells to methyl methanesulfonate. pC194-1 is distinct from pC194 in the addition of two A:T base pairs within the previously defined D region of pC194. Complementation experiments between pC194-1 and other plasmids suggest that the mutation of pC194-1 interferes with the production of a diffusible gene product required for plasmid maintenance.
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Alonso, J.C., Trautner, T.A. A gene controlling segregation of the Bacillus subtilis plasmid pC194. Mol Gen Genet 198, 427–431 (1985). https://doi.org/10.1007/BF00332934
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DOI: https://doi.org/10.1007/BF00332934