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A bacteriocinogenic factor of Enterobacter cloacae

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Summary

Enterobacter cloacae strain DF13 produces a bacteriocin which is able to kill other strains of Enterobacter and Klebsiella. This property can be transmitted to Enterobacter cloacae strain O 2 (up to 90% of the acceptor population became bacteriocinogenic), to E. coli K12F- and E. coli K 12 Hfr. Transfer of chromosome material was never observed, suggesting that the production of the bacteriocin is determined by a plasmid. However all attempts to eliminate this plasmid failed. The plasmid F′ trp cys Col B Col V could be transferred from E. coli into Ent. cloacae DF13 and subsequently it could be eliminated by acridine orange treatment. Ent. cloacae DF13 harbours in addition two independently transferable R-factors, one determining resistance against streptomycin and sulfanilamide and the other resistance against penicillin.

Most but not all Ent. cloacae O2 recombinants which have received only the bacteriocinogenic factor upon conjugation with Ent. cloacae DF 13, can transfer this property to Ent. cloacae O2 but not to E. coli. E. coli F- recombinants, which have received only the bacteriocinogenic factor cannot transfer this property. The results suggest that the bacteriocinogenic factor cannot mediate its own transfer, but can be transferred when another transmissible plasmid is present. This may be the R(str sul) factor, the F-factor in E. coli Hfr or a transfer factor (Δ) in Ent. cloacae O2.

Closed circular DNA molecules were selectively isolated from these strains and investigated by electron microscopy and velocity sedimentation. Ent. cloacae DF13 harbours small closed circular DNA molecules ranging from 0.5 to 3.2 μ in contour length, 98% of which corresponds to a size class of 0.7±0.1 μ. Ent. cloacae O2 also harbours closed circular DNA ranging from 0.8 to 3.0 μ in contour length, with major size classes of 0.9 μ and 1.4 μ respectively. Circular DNA of a contour length of 3.0±0.2 μ (S20,w=26 S) corresponding to a molecular weight of 6.0×106 daltons was transferred to E. coli and Ent. cloacae O 2 concomitantly with the ability to produce the bacteriocin. A significant difference was observed in the number of copies of the plasmid between Ent. cloacae and E. coli.

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References

  • Anderson, E. S.: Influence of the Δ transfer factor on the phage sensitivity of Salmonella. Nature (Lond.) 212, 795–799 (1966).

    Google Scholar 

  • —: The ecology of transferable drug resistance in the Enterobacteria. Ann. Rev. Microbiol. 22, 131–180 (1968).

    Google Scholar 

  • — Lewis, M. J.: Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Natur (Lond.) 208, 843–849 (1965).

    Google Scholar 

  • Asheshov, E. H.: Loss of antibiotic resistance in Staphylococcus aureus resulting from growth at high temperature. J. gen. Microbiol. 42, 403–410 (1966).

    Google Scholar 

  • Bazaral, M., Helinski, D. R.: Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J. molec. Biol. 36, 185–194 (1968).

    Google Scholar 

  • Bouanchaud, D. H., Scavizzi, M. R., Chabbert, Y. A.: Elimination by ethidiumbromide of antibiotic resistance in Enterobacteria and Staphylococci. J. gen. Microbiol. 54, 417–425 (1969).

    Google Scholar 

  • Clowes, R. C., Moody, E. E. H., Pritchard, R. H.: The elimination of extrachromosomal elements in thyminless strains of Escherichia coli K12. Gent. Res. 6, 147–152 (1965).

    Google Scholar 

  • Cozzarelli, N. R., Kelly, R. B., Kornberg, A.: A minute circular DNA from Escherichia coli 15. Proc. nat. Acad. Sci. (Wash.) 60, 992–999 (1968).

    Google Scholar 

  • Datta, N., Lawn, A. M., Meynell, E.: The relationship of F type piliation and F phage sensitivity to drug resistance transfer in R+ F- Escherichia coli K12. J. gen. Microbiol. 45, 365–376 (1966).

    Google Scholar 

  • Dubnau, E., Stocker, B. A. D.: Behavior of three colicine factors and an R-factor in Hfr crosses in Salmonella typhimurium. Genet. Res. 9, 283–297 (1967).

    Google Scholar 

  • Falkow, S., Citarella, R. V., Wohlhieter, J. A., Watanabe, T.: The molecular nature of R-factors. J. molec. Biol. 17, 102–116 (1966).

    Google Scholar 

  • Fredericq, P.: Colicins. Ann. Rev. Microbiol. 11, 7–24 (1957).

    Google Scholar 

  • —: Colicins and colicinogenic factors. Symp. Soc. exp. Biol. 12, 104–122 (1958).

    Google Scholar 

  • —: Colicines et autres bacteriocines. Ergebn. Mikrobiol. 37, 115–161 (1963).

    Google Scholar 

  • Freifelder, D.: Studies on Escherichia coli sex factors. IV. Molecular weights of the DNA of several F' factors. J. molec. Biol. 35, 95–102 (1968).

    Google Scholar 

  • Goebel, W., Helinski, D. R.: Generation of higher multiple circular DNA forms in bacteria. Proc. nat. Acad. Sci. (Wash.) 61, 1406–1413 (1968).

    Google Scholar 

  • Graaf, F. K., de, Tieze, G. A., Wendelaar Bonga, Sj., Stouthamer, A. H.: Purification and genetic determination of bacteriocin production in Enterobacter cloacae. J. Bact. 95, 631–640 (1968).

    Google Scholar 

  • Haan, P.G., de, Stouthamer, A. H., Felix, H. S., Mol, A. K.: Transfer of F' from Escherichia coli K12 to Escherichia coli B and to strains of Paracolobacter and Klebsiella. Antonie v. Leeuwenhoek 29,407–420 (1963).

    Google Scholar 

  • Hadjipetrou, L. P., Gerrits, J. P., Teulings, F. A. G., Stouthamer, A. H.: Relation between energy production and growth of Aerobacter aerogenes. J. gen. Microbiol. 36, 139–150 (1964).

    Google Scholar 

  • Hamon, Y., Péron, Y.: Etude d'une souche bacteriocinogène et lysogène d'Enterobacter aerogenes. Zbl. Bakt., I. Abt. Orig. 203, 184–189 (1967).

    Google Scholar 

  • Hayes, W.: The genetics of bacteria and their viruses, p. 747–748. Oxford and Edinburgh: Blackwell Sci. Publ. 1968.

    Google Scholar 

  • Hickson, F. T., Roth, Th. F., Helinski, D. R.: Circular DNA forms of a bacterial sex factor. Proc. nat. Acad. Sci. (Wash.) 58, 1731–1738 (1967).

    Google Scholar 

  • Hirota, Y.: The effect of acridine dyes on mating type factors in Escherichia coli K12. Proc. nat. Acad. Sci. (Wash.) 46, 57–64 (1968).

    Google Scholar 

  • Hudson, B., Clayton, D. A., Vinograd, J.: Complex mitochondrial DNA. Cold Spr. Harb. Symp. quant. Biol. 33, 435–442 (1968).

    Google Scholar 

  • Jansz, H. S., Pouwels, P. H., Schiphorst, J.: Preparation of double-stranded DNA (replicative form) of bacteriophage Φ X 174: a simplified method. Biochim. biophys. Acta (Amst.) 113, 626–627 (1966).

    Google Scholar 

  • — Zandberg, J., Pol, J. H. van de, Bruggen, E. F. J. van: Circulair DNA from Shigella paradysenteriae. Europ. J. Biochem. 9, 156–159 (1969).

    Google Scholar 

  • Lee, C. S., Davidson, N.: Covalently closed minicircular DNA in Micrococcus lysodeikticus. Biochem. biophys. Res. Commun. 32, 757–762 (1968).

    Google Scholar 

  • Mayne, E.: Essai d'elimination par l'acridine orange ou la mitomycine d'un facteur colicinogène lic- a un facteur de fertilité ches Escherichia coli. Ann. Inst. Pasteur 109, 154–157 (1965).

    Google Scholar 

  • Mitsuhashi, S., Hashimoto, H., Kono, M., Morimura, M.: Drug resistance of Staphylococci. II. Joint elimination and joint transduction of the determinants of penicillinase production and resistance to macrolide antibiotics. J. Bact. 89, 988–992 (1965).

    Google Scholar 

  • Mulczyk, M., Duguid, J. P.: Influence of the state of fimbriation on transmission of the colicinogenic factor Col I between strains of Shigella flexneri. J. gen. Microbiol. 45, 459–477 (1966).

    Google Scholar 

  • Nisioka, T., Mitani, M., Clowes, R. C.: Composite circular forms of R-factor DNA molecules. J. Bact. 97, 376–385 (1969).

    Google Scholar 

  • Nomura, M.: Colicins and related bacteriocins. Ann. Rev. Microbiol. 21, 257–284 (1967).

    Google Scholar 

  • Reeves, P.: The bacteriocins. Bact. Rev. 29, 24–45 (1965).

    Google Scholar 

  • Rownd, R., Nakaya, R., Nakamura, A.: Molecular nature of the drug resistance factors of the Enterobacteriaceae. J. molec. Biol. 17, 376–393 (1966).

    Google Scholar 

  • Smith, S. M., Ozeki, H., Stocker, B. A. D.: Transfer of Col E1 and Col E2 during high frequency transmission of Col I in Salmonella typhimurium. J. gen. Microbiol. 33, 231–242 (1963).

    Google Scholar 

  • Stouthamer, A. H., Haan, P. G. de, Bulten, E. J.: Kinetics of F-curing by acridine orange in relation to the number of F-particles in Eschrichia coli. Genet. Res. 4, 305–317 (1963).

    Google Scholar 

  • — Tieze, G. A.: Bacteriocin production by members of the genus Klebsiella. Antonie v. Leeuwenhoek 32, 171–182 (1966).

    Google Scholar 

  • Takahashi, I., Barnard, R. A.: Effect of N-methyl-N'-nitro-N-nitrosoguanidine on the F-factor of Escherichia coli. Mutation Res. 4, 111–117 (1967).

    Google Scholar 

  • Taylor, A. L., Trotter, C. D.: Revised linkage map of Escherichia coli. Bact. Rev. 31, 332–353 (1967).

    Google Scholar 

  • Tomoeda, M., Inuzuka, M., Kubo, N., Nakamura, S.: Effective elimination of drug resistance and sex factors in Escherichia coli by sodium dodecyl sulfate. J. Bact. 95, 1078–1089 (1968).

    Google Scholar 

  • Vinograd, J., Lebowitz, J., Watson, R.: Early and late helix-coil transitions in closed circular DNA. J. molec. Biol. 33, 173–187 (1968).

    Google Scholar 

  • Watanabe, T., Fukasawa, T.: Episome — mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors with acridine dyes. J. Bact. 81, 669–678 (1961).

    Google Scholar 

  • Willetts, N. S.: The elimination of Flac+ from E. coli by Mutagenic agents. Biochem. biophys. Res. Commun. 27, 112–117 (1967).

    Google Scholar 

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Authors and Affiliations

  1. Biological Laboratory, Microbiology Department, Free University, Amsterdam

    G. A. Tieze & A. H. Stouthamer

  2. Laboratory for Physiological Chemistry, State University, Utrecht

    H. S. Jansz & J. Zandberg

  3. Laboratory of Structural Chemistry, State University, Groningen, The Netherlands

    E. F. J. van Bruggen

Authors
  1. G. A. Tieze
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  2. A. H. Stouthamer
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  3. H. S. Jansz
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  4. J. Zandberg
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  5. E. F. J. van Bruggen
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Communicated by P. Starlinger

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Tieze, G.A., Stouthamer, A.H., Jansz, H.S. et al. A bacteriocinogenic factor of Enterobacter cloacae . Molec. Gen. Genetics 106, 48–65 (1969). https://doi.org/10.1007/BF00332820

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  • DOI: https://doi.org/10.1007/BF00332820

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