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Recombination and segregation of mitochondrial genes in Saccharomyces cerevisiae

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Summary

The results are given from a series of bifactorial crosses involving different combinations of one of a pair of non-allelic spiramycin resistance genes with one of a pair of non-allelic oligomycin resistance genes. The mating type of parental strains in a cross is found to influence the transmission frequency of these genes. For three of these genes the transmission frequency of the gene derived from the α parent is the complement of the transmission frequency of the gene derived from the a parent. The lack of such a complementary relationship for the transmission frequencies of the [OLG1] gene is discussed in terms of competition occurring between mitochondrial DNA molecules. The recombination percentage is found to be the same between any pair of spiramycin and oligomycin resistance genes and equal to 18%. However, for each pair the polarities of recombination are found to be different and possible explanations of this are discussed. The relative proportion of sensitive cells at a particular time in a cross is found to be characteristic for a mitochondrial gene. A model to provide a basis for such an observation also predicts that such values can be used in the mapping and ordering of mitochondrial genes.

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  1. Department of Genetics, University of Adelaide, South Australia

    David F. Callen

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  1. David F. Callen
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Communicated by F. Kaudewitz

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Callen, D.F. Recombination and segregation of mitochondrial genes in Saccharomyces cerevisiae . Molec. gen. Genet. 134, 49–63 (1974). https://doi.org/10.1007/BF00332812

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