Skip to main content
SpringerLink
Log in

An unlinked mutation affecting control of purine metabolism in a revertant of an ad-7 auxotroph of Neurospora crassa lacking the phosphoribosylpyrophosphate amidotransferase

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Prototrophic revertants of an ad-7 mutant recover phosphoribosylpyrophosphate amidotransferase activity, indicating ad-7 is the structural gene for the first enzyme in the de novo biosynthesis of purine nucleotides. Some revertants excrete purines and cross-feed ad-3A conidia which have a lesion in the de novo pathway. A mutant apu, not linked to ad-7 or ad-8, is responsible for the ability to excrete purines. This mutation is not allelic to a mutant in the aza-1 locus which is also known to excrete purines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bernstein, H.: Imidazole compounds accumulated by purine mutants of Neurospora crassa. J. gen. Microbiol. 25, 41–46 (1961).

    Google Scholar 

  • Buchanan, J. M.: The enzymatic synthesis of the purine nucleotides. Harvey Lectures, 1958–1959. Series LIV, p. 104–130. New York-London: Academic Press 1960.

    Google Scholar 

  • Flaks, J. L., Lukens, L. N.: The enzymes of purine nucleotide synthesis de novo In: S. P. Colowick and N. O. Kaplan (eds.), Methods in enzymology, vol. VI, p. 52–95. New York: Academic Press 1963.

    Google Scholar 

  • Ishikawa, T.: Genetic studies of ad-8 mutants in Neurospora crassa. I. Genetic fine structure of the ad-8 locus. Genetics 47, 1147–1161 (1962).

    Google Scholar 

  • Jha, K. K.: Genes conferring resistance to 8-aza adenine in Neurospora crassa and the variability of resistant alleles in the aza-1 locus with respect to excretion of purines. Molec. gen. Genet. 114, 156–167 (1971).

    Google Scholar 

  • Vogel, H. J.: Distribution of lysine pathways among fungi: evolutionary implications. Amer. Naturalist 98, 435–446 (1964).

    Google Scholar 

  • Westergaard, M., Mitchell, H. K.: Neurospora. V. A synthetic medium favouring sexual reproduction. Amer. J. Bot. 34, 573–577 (1947).

    Google Scholar 

  • Wyngaarden, J. B., Ashton, D. M.: The regulation of activity of phosphoribosylpyrophosphate amidotransferase by purine ribonucleotides: a potential feedback control of purine biosynthesis. J. biol. Chem. 234, 1492–1496 (1959).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, Research School of Biological Sciences, Australian National University, Canberra, Australia

    Krishna Kumar Jha

  2. Department of Botany, University of North Carolina, 27514, Chapel Hill, N. C., USA

    Krishna Kumar Jha

Authors
  1. Krishna Kumar Jha
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gajewski

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kumar Jha, K. An unlinked mutation affecting control of purine metabolism in a revertant of an ad-7 auxotroph of Neurospora crassa lacking the phosphoribosylpyrophosphate amidotransferase. Molec. Gen. Genetics 114, 168–172 (1972). https://doi.org/10.1007/BF00332787

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00332787

Keywords

Search

Navigation