Summary
The number of specific protein binding sites on 23S RNA were determined using the same conditions and methods as those described in the previous paper. Eight proteins out of thirty-four 50S subunit proteins, namely L2, L6, L16, L17, L19, L20, L23 and L24, were found to bind specifically to the 23S RNA. Each of the proteins bound simultaneously at about a 1:1 stoichiometric molar ratio and exclusively with 23S rRNA in the presence of 16S rRNA.
Similar content being viewed by others
References
Crichton, R. R., Wittmann, H. G.: Ribosomal proteins. XXIV. Trypsin digestion as a probe of the conformation of Escherichia coli ribosomes. Molec. gen. Genet. 114, 95–105 (1971).
Garrett, R.A., Rak, K.H., Daya, L., Stöffler, G.: Ribosomal proteins. XXIX. Specific protein binding sites on 16S RNA of Escherichia coli. Molec. gen. Genet. 114, 112–124 (1971).
Hardy, S.J.S., Kurland, C.G., Voynow, P., Mora, G.: The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry 8, 2897–2905 (1969).
Hindennach, I., Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins. X. Isolation of proteins from 50S ribosomal subunits of Escherichia coli. 23, 12–16 (1971).
Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins. VII. Two dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analyt. Biochem. 36, 401–412 (1970).
Mizushima, S., Nomura, M.: Assembly mapping of 30S ribosomal proteins from Escherichia coli. Nature (Lond.) 226, 1214–1218 (1970).
Nomura, M., Erdmann, V. A.: Reconstitution of 50S ribosomal subunits from dissociated molecular components. Nature (Lond.) 228, 744–748 (1970).
Ouchterlony, O.: Diffusion in gel methods for immunological analyses. Progr. Allergy 5, 1 (1958).
Staehelin, T., Maglott, D., Monro, R.E.: On the catalytic centre of peptidyl transfer: A part of the 50S ribosome structure. Cold Spr. Harb. Symp. quant. Biol. 34, 39–48 (1969).
Stöffler, G.: Immunologische Untersuchungen mit Antiseren genen isolierte ribosomale Proteine aus 30S und 50S Untereinheiten aus Escherichia coli. Hoppe-Seylers Z. physiol. Chem. 350, 116 (1969).
Stöffler, G., Daya, L., Rak, K.H., Garrett, R.A.: Ribosomal proteins XXVI. The number of specific protein binding sites on 16S and 23S RNA of Escherichia coli. J. molec. Biol. 62, 411–414 (1971).
—, Wittmann, H.G.: Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods. Proc. nat. Acad. Sci. (Wash.) 68, 2283–2287 (1971).
Stöffler, G., Wittmann, H.G.: Ribosomal proteins. XXV. Immunological studies on Escherichia coli ribosomal proteins. J. molec. Biol. (in press).
Traub, P., Nomura, M.: Structure and function of Escherichia coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and protein. Proc. nat. Acad. Sci. (Wash.) 59, 777–784 (1968).
—: Structure and function of Escherichia coli ribosomes. VI. Mechanisms of assembly of 30S ribosomes studied in vitro. J. molec. Biol. 40, 391–413 (1969).
Author information
Authors and Affiliations
Additional information
Communicated by P. Starlinger
Rights and permissions
About this article
Cite this article
Stöffler, G., Daya, L., Rak, K.H. et al. Ribosomal proteins. Molec. Gen. Genetics 114, 125–133 (1972). https://doi.org/10.1007/BF00332783
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332783