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Ribosomal proteins

XXX. Specific protein binding sites on 23S RNA of Escherichia coli

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Summary

The number of specific protein binding sites on 23S RNA were determined using the same conditions and methods as those described in the previous paper. Eight proteins out of thirty-four 50S subunit proteins, namely L2, L6, L16, L17, L19, L20, L23 and L24, were found to bind specifically to the 23S RNA. Each of the proteins bound simultaneously at about a 1:1 stoichiometric molar ratio and exclusively with 23S rRNA in the presence of 16S rRNA.

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References

  • Crichton, R. R., Wittmann, H. G.: Ribosomal proteins. XXIV. Trypsin digestion as a probe of the conformation of Escherichia coli ribosomes. Molec. gen. Genet. 114, 95–105 (1971).

    Google Scholar 

  • Garrett, R.A., Rak, K.H., Daya, L., Stöffler, G.: Ribosomal proteins. XXIX. Specific protein binding sites on 16S RNA of Escherichia coli. Molec. gen. Genet. 114, 112–124 (1971).

    Google Scholar 

  • Hardy, S.J.S., Kurland, C.G., Voynow, P., Mora, G.: The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry 8, 2897–2905 (1969).

    Google Scholar 

  • Hindennach, I., Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins. X. Isolation of proteins from 50S ribosomal subunits of Escherichia coli. 23, 12–16 (1971).

    Google Scholar 

  • Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins. VII. Two dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analyt. Biochem. 36, 401–412 (1970).

    Google Scholar 

  • Mizushima, S., Nomura, M.: Assembly mapping of 30S ribosomal proteins from Escherichia coli. Nature (Lond.) 226, 1214–1218 (1970).

    Google Scholar 

  • Nomura, M., Erdmann, V. A.: Reconstitution of 50S ribosomal subunits from dissociated molecular components. Nature (Lond.) 228, 744–748 (1970).

    Google Scholar 

  • Ouchterlony, O.: Diffusion in gel methods for immunological analyses. Progr. Allergy 5, 1 (1958).

    Google Scholar 

  • Staehelin, T., Maglott, D., Monro, R.E.: On the catalytic centre of peptidyl transfer: A part of the 50S ribosome structure. Cold Spr. Harb. Symp. quant. Biol. 34, 39–48 (1969).

    Google Scholar 

  • Stöffler, G.: Immunologische Untersuchungen mit Antiseren genen isolierte ribosomale Proteine aus 30S und 50S Untereinheiten aus Escherichia coli. Hoppe-Seylers Z. physiol. Chem. 350, 116 (1969).

    Google Scholar 

  • Stöffler, G., Daya, L., Rak, K.H., Garrett, R.A.: Ribosomal proteins XXVI. The number of specific protein binding sites on 16S and 23S RNA of Escherichia coli. J. molec. Biol. 62, 411–414 (1971).

    Google Scholar 

  • —, Wittmann, H.G.: Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods. Proc. nat. Acad. Sci. (Wash.) 68, 2283–2287 (1971).

    Google Scholar 

  • Stöffler, G., Wittmann, H.G.: Ribosomal proteins. XXV. Immunological studies on Escherichia coli ribosomal proteins. J. molec. Biol. (in press).

  • Traub, P., Nomura, M.: Structure and function of Escherichia coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and protein. Proc. nat. Acad. Sci. (Wash.) 59, 777–784 (1968).

    Google Scholar 

  • —: Structure and function of Escherichia coli ribosomes. VI. Mechanisms of assembly of 30S ribosomes studied in vitro. J. molec. Biol. 40, 391–413 (1969).

    Google Scholar 

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Communicated by P. Starlinger

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Stöffler, G., Daya, L., Rak, K.H. et al. Ribosomal proteins. Molec. Gen. Genetics 114, 125–133 (1972). https://doi.org/10.1007/BF00332783

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