Summary
The gene for Escherichia coli ribosomal protein S15 (rpsO) was cloned on the vector pBR322 from F-prime JCH55 DNA. The recombinant plasmid was transformed to Serratia marcescens cells and it was proved that E. coli S15 was synthesized and incorporated into ribosome particles in S. marcescens cells. A DNA fragment containing rpsO was also inserted into the vector pRF3, which changes its copy number depending on the growth temperature in a temperature-sensitive polA host. By use of this recombinant plasmid it was shown that the relative synthesis rate of S15 increased about twice even when the copy number of the plasmid increased more than twenty-fold.
Similar content being viewed by others
References
Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12. Microbiol Rev 44:1–56
Brot N, Caldwell P, Weissbach H (1980) Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro. Proc Natl Acad Sci USA 77:2592–2595
Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics (A manual for genetic engineering). Cold Spring Harbor Laboratory, New York, pp 116–119
Dean D, Yates JL, Nomura M (1981) Identification of ribosomal protein S7 as a repressor of translation within the str operon of E. coli. Cell 24:413–419
Fukuda R (1980) Autogenous regulation of the synthesis of ribosomal proteins, L10 and 17/12, in Escherichia coli. Mol Gen Genet 178:483–486
Kaltschmidt E, Wittmann HG (1970) Ribosomal proteins. VII. Two-dimensional polyaclylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem 36:401–412
Kitakawa M, Dabbs ER, Isono K (1979) Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli. J Bacteriol 138:832–838
Komai N, Nishizawa P, Hayakawa Y, Murotsu T, Matsubara K (1982) Detection and mapping of sex miniF encoded protein by cloning analysis of dissected miniF segment. Mol Gen Gent (in press)
Lee JS, An G, Friesen JD, Isono K (1981) Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rpmG). Mol Gen Genet 184:218–223
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York, pp 431–433
Nomura M, Held WA (1974) Reconstitution of ribosomes: Studies of ribosome structure, function and assembly. In: Nomura M, Tissières A, Lengyel P (eds) Ribosomes. Cold Spring Harbor Laboratory, New York, pp 193–223
Nomura M, Yates JL, Dean D, Post LE (1980) Feedback regulation of ribosomal protein gene expression in Escherichia coli: Structural homology of ribosomal RNA and ribosomal protein mRNA. Proc Natl Acad Sci USA 77:7084–7088
Ohtsubo E (1970) Transfer-defective mutants of sex factors in Escherichia coli. II. Deletion mutants of an F-prime and deletion mapping of cistrons involved in genetic transfer. Genetics 64:189–197
Reid JD, Stoufer SD, Ogrydziak DM (1982) Efficient transformation of Serratia marcescens with pBR322 plasmid DNA. Gene 17:107–112
Takata R (1978) Genetic studies of the ribosomal proteins in Escherichia coli XI. Mapping of the genes for L21, L27, S15 and S21 by using hybrid bacteria and over-production of these proteins in the merodiploid strains. Mol Gen Genet 160:151–155
Tsurimoto T, Matsubara K (1980) Preparation of plasmid DNA. Metabolism (Japanese) 17:81–89
Yates JL, Arfsten AE, Nomura M (1980) In vitro expression of Escherichia coli ribosomal protein genes: Autogenous inhibition of translation. Proc Natl Acad Sci USA 77:1837–1841
Yates JL, Nomura M (1980) E. coli robosomal protein L4 is a feedback regulatory protein. Cell 21:517–522
Yates JL, Nomura M (1981) Feedback regulation of ribosomal protein synthesis in E. coli: Localization of the mRNA target sites for repressor action of ribosomal protein L1. Cell 24:243–249
Author information
Authors and Affiliations
Additional information
Communicated by K. Isono
Rights and permissions
About this article
Cite this article
Takata, R., Aoyagi, M. & Mukai, T. Cloning of rpsO, the gene for ribosomal protein S15 of Escherichia coli . Mol Gen Genet 188, 334–337 (1982). https://doi.org/10.1007/BF00332697
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332697