Skip to main content
Log in

Inverted duplication in the genome of the temperate Streptomyces phage SH3

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The DNA of the temperate Streptomyces phage SH3 contains 100 base-pair long inverted repeats separated by a 940 base-pair long segment of DNA as revealed by electromicroscopic analysis of snapback structures formed after rapid intrastrand reannealing of denatured DNA. The inverted repeat structure was found preferentially at map unit 22 of the circular physical map, in rare cases also in other positions, suggesting a movable character of this genetic element.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Arber W, Iida S, Jütte H, Caspers P, Meyer J, Hänni C (1979) Rearrangements of genetic material in Escherichia coli as observed on the bacteriophage P1 plasmid. Cold Spring Harbor Symp Quant Biol 43:1197–12098

    Google Scholar 

  • Beck E, Sommer R, Auerswald EA, Kurz C, Zink B, Osterburg G, Schaller H, Sugimoto K, Sugisaki H, Okamoto T, Takanami M (1978) Nucleotide sequence of bacteriophage fd DNA. Nucl Acids Res 5:4495–4503

    Google Scholar 

  • Bukhari AI, Shapiro JA, Adhya SL (1977) DNA insertion, elements, plasmids, and episomes. Cold Spring Harbor Laboratory, New York

    Google Scholar 

  • Campbell A, Berg D, Botstein D, Lederberg E, Novick R, Starlinger P, Szybalski W (1977) Nomenclature of transposable elements in prokaryotes. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, New York, pp 15–22

    Google Scholar 

  • Chater KF, Hopwood DA (1982) Streptomyces genetics. In: Goodfellow M, Modarski M, Williams ST (eds) The Actinomycetales. Academic Press, New York London, pp

    Google Scholar 

  • Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Grossman L, Moldave K (eds) Methods in enzymology. Academic Press, New York, pp 413–428

    Google Scholar 

  • Iida S, Meyer J, Arber W (1978) The insertion element IS1 is a natural constituent of coliphage P1 DNA. Plasmid 1:357–365

    Google Scholar 

  • Iida S, Meyer J, Arber W (1981) Genesis and natural history of IS-mediated transposons. Cold Spring Harbor Symp Quant Biol 45:27–37

    Google Scholar 

  • Klaus S, Triebel H, Hartmann M, Walter A, Walter F, Zöpel P, Bär H (1979) Molecular characterization of the genomes of actinophages SH3, SH10, SH11, and SH12 infecting Streptomyces hygroscopicus. Mol Gen Genet 172:319–327

    Google Scholar 

  • Nakano MM, Ogawara H (1980) Multiple effects induced by unstable mutation in Streptomyces lavendulae. J Antibiot 33:420–425

    Google Scholar 

  • Rautenstein JI, Solvyeva NJ, Roth M, Noack D, Klaus S (1977) Lysogeny of the culture of Streptomyces hygroscopicus producing the antibiotic turimycin and some characteristics of its temperate phage. Mikrobiologia (in Russian) 45:922–924

    Google Scholar 

  • Redshaw PA, McCann PA, Pentella MA, Pogell BM (1979) Simultaneous loss of multiple differentiation functions in aerial mycelium-negative isolates of Streptomycetes. J Bacteriol 137:891–899

    Google Scholar 

  • Roth M, Noack D, Solovyeva NJ, Rautenstein JI (1978) Effective enrichment of the temperate actinophage SH3 and its interaction with Streptomyces hygroscopicus. Z Allgem Mikrobiol 18:147–149

    Google Scholar 

  • Roth M, Noack D (1982) Genetic stability of differentiated functions in Streptomyces hygroscopicus in relation to conditions of continuous culture. J Gen Microbiol 128:107–114

    Google Scholar 

  • Sladkova IA, Chinenova TA, Vasilchenko LG, Pelts LB, Lomovskaya ND (1980) Physical mapping of Streptomyces coelicolor A3(2) actinophages. II. A transposonlike structure in ΦC43 DNA molecules and location of the region responsible for establishment of lysogenic state. Mol Biol 14:916–921

    Google Scholar 

  • Starlinger P, Saedler H (1976) IS-elements in microorganisms. Curr Top Microbiol Immunol 75:111–152

    Google Scholar 

  • Sutcliffe EM (1979) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol 43:77–90

    Google Scholar 

  • Walter F, Hartmann M, Klaus S (1981) Restriction endonuclease analysis of DNA from the Streptomyces phages SH3, SH5, SH10, and SH13. Gene 13:57–63

    Google Scholar 

  • Walter F, Hartmann M, Klaus S (in preparation) Studies on the packaging constraints of Streptomyces phage SH3

  • Westmoreland BC, Szybalski W, Ris H (1969) Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy. Science 163:1343–1348

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by H. Böhme

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klaus, S., Hartmann, M., Walter, F. et al. Inverted duplication in the genome of the temperate Streptomyces phage SH3. Mol Gen Genet 188, 322–324 (1982). https://doi.org/10.1007/BF00332695

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00332695

Keywords

Navigation