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Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae

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Summary

Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD + strains; only 30–60% of pyrimidine dimers and 25–40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.

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References

  • Cole RS, Levitan D, Sinden RR (1976) Removal of psoralen interstrand crosslinks from DNA of Escherichia coli: mechanism and genetic control. J Mol Biol 103:39–59

    Google Scholar 

  • Martin P, Prakash L, Prakash S (1981) a/α-Specific effect of the mms3 mutation on ultraviolet mutagenesis in Saccharomyces cerevisiae. J Bacteriol 146:684–691

    Google Scholar 

  • McKnight GL, Cardillo TS, Sherman F (1981) An extensive deletion causing overproduction of yeast iso-2-cytochrome c. Cell 25:409–419

    Google Scholar 

  • Miller RD, Prakash L, Prakash S (1982) Genetic control of excision of Saccharomyces cerevisiae interstrand DNA cross-links induced by psoralen plus near-UV light. Mol Cell Biol 2:939–948

    Google Scholar 

  • Mortelmans K, Friedberg EC, Slor H, Thomas G, Cleaver JE (1976) Defective thymine dimer excision by cell-free extracts of xeroderma pigmentosum cells. Proc Natl Acad Sci USA 73:2757–2761

    Google Scholar 

  • Prakash L (1975) Repair of pyrimidine dimers in nuclear and mitochondrial DNA of yeast irradiated with low doses of ultraviolet light. J Mol Biol 98:781–795

    Google Scholar 

  • Prakash L (1977a) Repair of pyrimidine dimers in radiation sensitive mutants rad3, rad4, rad6 and rad9 of Saccharomyces cerevisiae. Mutat Res 45:13–20

    Google Scholar 

  • Prakash L (1977b) Defective thymine dimer excision in radiationsensitive mutants rad10 and rad16 of Saccharomyces cerevisiae. Mol Gen Genet 162:125–128

    Google Scholar 

  • Prakash L, Prakash S (1979) Three additional genes involved in pyrimidine dimer removal in Saccharomyces cerevisiae: RAD7, RAD14 and MMS19. Mol Gen Genet 176:351–359

    Google Scholar 

  • Resnick MA, Setlow JK (1972) Repair of pyrimidine dimer damage induced in yeast by ultraviolet light. J Bacteriol 109:979–986

    Google Scholar 

  • Reynolds RJ (1978) Removal of pyrimidine dimers from Saccharomyces cerevisiae nuclear DNA under nongrowth conditions as detected by a sensitive enzymatic assay. Mutat Res 50:43–56

    Google Scholar 

  • Reynolds RJ, Friedberg EC (1981) Molecular mechanisms of pyrimidine dimer excision in Saccharomyces cerevisiae. Incision of ultraviolet-irradiated deoxyribonucleic acid in vivo. J Bacteriol 146:692–704

    Google Scholar 

  • Seeberg E (1981) Strand cleavage at psoralen adducts and pyrimidine dimers in DNA cansed by interaction between semipurified uvr + gene products from Escherichia coli. Mutat Res 82:11–22

    Google Scholar 

  • Sherman F, Slonimski PP (1964) Respiration-deficient mutants of yeast. II. Biochemistry. Biochim Biophys Acta 90:1–15

    Google Scholar 

  • Singh A, Sherman F (1978) Deletions of the iso-1-cytochrome c and adjacent genes of yeast: discovery of the OSM1 gene controlling osmotic sensitivity. Genetics 89:653–665

    Google Scholar 

  • Unrau P, Wheatcroft R, Cox BS (1971) The excision of pyrimidine dimers from DNA of ultraviolet irradiated yeast. Mol Gen Genet 113:359–362

    Google Scholar 

  • Waters R, Moustacchi E (1974) The disappearance of ultravioletinduced pyrimidine dimers from the nuclear DNA of exponential and stationary phase cells of Saccharomyces cerevisiae following various post irradiation treatments. Biochim Biophys Acta 353:407–419

    Google Scholar 

  • Wilcox DR, Prakash L (1981) Incision and postincision steps of pyrimidine dimer removal in excision-defective mutants of Saccharomyces cerevisiae. J Bacteriol 148:618–623

    Google Scholar 

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Authors and Affiliations

  1. Department of Radiation Biology and Biophysics, University of Rochester School of Medicine, 14642, Rochester, NY, USA

    Raymond D. Miller & Louise Prakash

  2. Department of Biology, University of Rochester, 14627, Rochester, NY, USA

    Satya Prakash

Authors
  1. Raymond D. Miller
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  2. Louise Prakash
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  3. Satya Prakash
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Communicated by B.A. Bridges

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Miller, R.D., Prakash, L. & Prakash, S. Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae . Mol Gen Genet 188, 235–239 (1982). https://doi.org/10.1007/BF00332681

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  • DOI: https://doi.org/10.1007/BF00332681

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