Summary
Methods are presented for the recovery of separate fractions of rabbit submaxillary acinar and striated duct cells from acini and duct segments, by means of density gradient centrifugation in glycerol-sucrose mixtures.
Incomplete acinar fragmentation discloses the presence of acellular, nonstaining interacinar structures, referred to as interacinar bridges. The effectiveness of trypsin in liberating the acinar cells is ascribed to the disruption of intercellular secretory capillaries and interacinar bridges.
The basal processes of the isolated striated duct cells correspond with both the striations observed with optical microscopy and the infolded cytoplasmic compartments revealed with electron microscopy. The presence of clearly discernible spaces between the basal processes is compatible with the concept of intermembrane dilations.
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Schneider, R.M. Observations on the morphology of submaxillary gland cells isolated in a density gradient, and the demonstration of interacinar bridges. Zeitschrift für Zellforschung 57, 838–847 (1962). https://doi.org/10.1007/BF00332466
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DOI: https://doi.org/10.1007/BF00332466