Summary
Two- and three-point extranuclear crosses have been carried out via heterokaryons involving the three extranuclear mitochondrial markers of Aspergillus nidulans: (oliA1), (cs67) and (camA112). All three markers appear to be located on a single functional mitochondrial genome. Recombination between all three pairs of extranuclear markers appears to be equally frequent, suggesting a lack of genetic linkage. An important feature of these results is the variable and often marked non-equality of frequency of reciprocal classes of recombinants.
Similar content being viewed by others
References
Adoutte, A.: Mitochondrial mutations in Paramecium: phenotypical characterisation and recombination. In: The biogenesis of mitochondria. Transcriptional, translational and genetic aspects (Kroon, A. M., Saccone, C., eds.), p. 263–271. Proceedings of the International Conference on the Biogenesis of Mitochondria, Bari, Italy. New York and London: Academic Press Inc. 1974
Avner, P. R., Coen, D., Dujon, B., Slonimski, P. P.: Mitochondrial genetics. IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae. Molec. gen. Genet. 125, 9–52 (1973)
Callen, D. F.: Recombination and segregation of mitochondrial genes in Saccharomyces cerevisiae. Molec. gen. Genet. 134, 49–63 (1974)
Coen, D., Deutsch, J., Netter, P., Petrochilo, E., Slonimski, P. P.: Mitochondrial genetics. I. Methodology and phenomenology. In: Control of organelle development. Symp. Soc. exp. Biol. 24, 449–496 (1970)
Gouhier-Monnerot, M.: Ethidium bromide resistance and enhancement of mitochondrial recombination. Molec. gen. Genet. 130, 65–79 (1974)
Gunatilleke, I. A. U. N., Scazzocchio, C., Arst, Jr., H. N.: Cytoplasmic and nuclear mutations to chloramphenicol resistance in Aspergillus nidulans. Molec. gen. Genet. 137, 269–276 (1975)
Howell, N., Hall, R. M., Linnane, A. W., Lukins, H. B.: Genetic analyses of the polarity alleles in recombinants from mitochondrial genetic crosses. J. Bact. 119, 1063–1065 (1974)
Howell, N., Trembath, M. K., Linnane, A. W., Lukins, H. B.: Biogenesis of mitochondria. 30. An analysis of polarity of mitochondrial gene recombination and transmission. Molec. gen. Genet. 122, 37–51 (1973)
Lancashire, W. E., Griffiths, D. E.: Studies on energy-linked reactions: genetic analysis of venturicidin-resistant mutants. Europ. J. Biochem. 51, 403–413 (1975)
Rank, G. H.: Recombination in 3-factor crosses of cytoplasmically inherited antibiotic-resistance mitochondrial markers in S. cerevisiae. Heredity 30, 265–271 (1973)
Rowlands, R. T.: Extranuclear mutants of Aspergillus nidulans. Ph. D. Thesis, University of Bristol, England, U.K. (1974)
Rowlands, R. T., Turner, G.: Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. Molec. gen. Genet. 126, 201–216 (1973)
Rowlands, R. T., Turner, G.: Physiological and biochemical studies of nuclear and extranuclear oligomycin-resistant mutants of Aspergillus nidulans. Molec. gen. Genet. 132, 73–88 (1974a)
Rowlands, R. T., Turner, G.: Recombination between the extranuclear genes conferring oligomycin resistance and cold sensitivity in Aspergillus nidulans. Molec. gen. Genet. 133, 151–161 (1974b)
Sager, R., Ramanis, Z.: A genetic map of non-Mendelian genes in Chlamydomonas. Proc. nat. Acad. Sci. (Wash.) 65, 593–600 (1970)
Thomas, D. Y., Wilkie, D.: Recombination of mitochondrial drug-resistance factors in Saccharomyces cerevisiae. Biochem. biophys. Res. Commun. 30, 368–372 (1968)
Turner, G., Rowlands, R. T.: Cytochrome abnormalities and cyanide-resistant respiration in extranuclear mutants of Aspergillus nidulans. J. Bacteriol. submitted for publication
Waldron, C., Roberts, C. F.: Cytoplasmic inheritance of a cold-sensitive mutant in Aspergillus nidulans. J. gen. Microbiol. 78, 379–381 (1973)
Waxman, M. F., Eaton, N., Wilkie, D.: Effect of antibiotics on the transmission of mitochondrial factors in Saccharomyces cerevisiae. Molec. gen. Genet. 127, 277–284 (1973)
Wolf, K., Dujon, B., Slonimski, P. P.: Mitochondrial genetics. V. Multifactorial mitochondrial crosses involving a mutation conferring paromomycin-resistance in Saccharomyces cerevisiae. Molec. gen. Genet. 125, 53–90 (1973)
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Rowlands, R.T., Turner, G. Three-marker extranuclear mitochondrial crosses in Aspergillus nidulans . Molec. gen. Genet. 141, 69–79 (1975). https://doi.org/10.1007/BF00332379
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332379