Summary
When λ lysogens of E. coli are induced by γ-radiation the λ repressor, as measured by its specific binding to λ DNA, is rapidly inactivated by a recA +-dependent process which does not require new protein synthesis. This rapid inactivation is similar to inactivation of repressor by expression of the temperature sensitive E. coli mutation tif. In contrast, induction by UV irradiation or mitomycin C treatment requires new protein synthesis and there is a lag before the repressor is inactivated (Tomizawa and Ogawa, 1967; Shinagawa and Itoh, 1973).
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Beukers, R., Ijlstra, J., Berends, W.: The effect of ultraviolet light on some compounds of the nucleic acids, VI. The origin of the ultraviolet sensitivity of deoxyribonucleic acid. Rec. Trav. Chem. 79, 101–104 (1960)
Brooks, K., Clark, A. J.: Behaviour of λ bacteriophage in a recombination deficient strain of Escherichia coli. J. Virol. 1, 283–293 (1967)
Castellazzi, M., George, J., Buttin, G.: Prophage induction and cell division in E. coli: 1. Further characterisation of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation. Molec. gen. Genet. 119, 139–152 (1972a)
Castellazzi, M., George, J., Buttin, G.: Prophage induction and cell division in E. coli: 2. Linked (recA, zab) and unlinked (lex) suppressors of tif-1 mediated induction and filamentation. Molec. gen. Genet. 119, 153–174 (1972b)
Echols, H., Green, L.: Establishment and maintenance of repression by bacteriophage lambda: The role of the cI, cII and cIII proteins. Proc. nat. Acad. Sci. (Wash.), 68, 2190–2194 (1971)
Emmerson, P. T.: X-ray damage to DNA and loss of biological function: Effect of sensitising agents. Adv. Rad. Chem. 3, 209–270 (1972)
Howard-Flanders, P., Theriot, L.: Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics 53, 1137–1150 (1966)
Iyer, I. N., Szybalski, W.: A molecular mechanism of mitomycin action: linking of complementary DNA strands. Proc. nat. Acad. Sci. (Wash) 50, 355–362 (1963)
Johansen, I., Brustad, T., Rupp, W. D.: DNA strand breaks measured within 100 msecs of irradiation of E. coli by 4 MeV electrons. Proc. nat. Acad. Sci. (Wash.), 72, 167–171 (1975)
Kaiser, A. D.: Mutations in a temperate bacteriophage affecting its ability to lysogenise E. coli. Virology 3, 42–61 (1957)
Kirby, E. P., Jacob, F., Goldthwait, D. A.: Prophage induction and filament formation in a mutant strain of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 58, 1903–1910 (1967)
Latarjet, R.: Induction, par les rayon X, de la production d'un bactériophage chez Bacillus megatherium lysogène. Ann. Inst. Pasteur, 81, 389–393 (1951)
Lwoff, A., Siminovitch, L., Kjeldgaard, N.: Induction de la production de bactériophages chez une bactérie lysogène. Ann. Inst. Pasteur 79, 815–859 (1950)
Marcovich, H.: Etude radiobiologique du système lysogène d'Escherichia coli K12: 1. Rayons X. Ann. Inst. Pasteur 90, 303–319 (1956)
Mattern, I.E., van Winden, M. P., Rörsch, A.: The range of action of genes controlling radiation sensitivity in Escherichia coli. Mutation Res. 2, 111–131 (1965)
Monod, J., Cohen-Bazire, G., Cohn, M.: Sur la biosynthese de la β galactosidase (lactase) chez Escherichia coli. La specificite de l'induction. Biochem. biophys. Acta (Amst.) 7, 585–599 (1951)
Ptashne, M.: Repressor and its action. In: The Bacteriophage Lambda, (Hershey, A. D. ed.), p. 221–245. Cold Spring Harbor Laboratory, N. Y. (1971)
Reichardt, L., Kaiser, A. D.: Control of repressor synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 2185–2189 (1971)
Roberts, J. W., Roberts, C. W.: Proteolytic cleavage of bacteriophage lambda repressor in induction. Proc. nat. Acad. Sci. (Wash.) 72, 147–151 (1975)
Shimada, K., Ogawa, H., Tomizawa, J.: Studies on radiation sensitive mutants of E. coli. II Breakage and repair of ultraviolet irradiated intracellular DNA of phage lambda. Molec. gen. Genet. 101, 245–256 (1968)
Shinagawa, H., Itoh, T.: Inactivation of DNA binding activity of repressor in extracts of lambda lysogens treated with Mitomycin C. Molec. gen. Genet. 126, 103–110 (1973)
Sussman, R., Jacob, F.: Sur un système de répression thermosensible chez le bacteriophage λ d'Escherichia coli. C. R. Acad. Sci. (Paris) 254, 1517 (1962)
Tomizawa, J., Ogawa, T.: Effect of UV irradiation on bacteriophage lambda immunity. J. molec. Biol. 23, 247–263 (1967)
Wacker, A., Dellweg, H., Weinblum, D.: Strahlenchemische Veränderung der Bakterien Desoxyribonucleinsaure in vivo. Naturwissenschaften 47, 477–480 (1960)
Wu, A. M., Ghosh, S., Echols, H., Spiegelman, W. G.: Repression of the c1 protein of phage λ in vitro inhibition of RNA synthesis. J. mol. Biol. 67, 407–421 (1972)
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Communicated by B. A. Bridges
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West, S.C., Powell, K.A. & Emmerson, P.T. recA +-dependent inactivation of the lambda repressor in Escherichia coli lysogens by γ-radiation and by tif expression. Molec. gen. Genet. 141, 1–8 (1975). https://doi.org/10.1007/BF00332374
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DOI: https://doi.org/10.1007/BF00332374