Summary
DNA replication of lambda phage depends on transcriptional activation at or around the λori region by RNA polymerase. To elucidate the function of the transcriptional activation, we constructed several plasmids carrying λori and lacP, whose relative locations and directions were different from each other, and studied replication activity of these recombinant plasmids. Transcription in a region immediately downstream from λori, but not in the λori region, was found to be essential for plasmid replication. Transcription proceeding over a certain minimal length was required and only rightward-directed transcription was effective for the activation
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Abbreviations
- Apr :
-
ampicillin resistance
- Cmr :
-
chloramphenicol resistance
- Smr :
-
streptomycin resistance
- IPTG:
-
Isopropyl-β-D-thiogalactoside
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Communicated by M. Takanami
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Hase, T., Nakai, M. & Masamune, Y. Transcription of a region downstream from λ ori is required for replication of plasmids derived from coliphage lambda. Mol Gen Genet 216, 120–125 (1989). https://doi.org/10.1007/BF00332239
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DOI: https://doi.org/10.1007/BF00332239