Summary
The conjugative behaviour of nopaline and agropine Ti-plasmids has been investigated. Using a technique which avoids enrichment of transconjugants on a mating medium we have shown that preculture in the presence of agrocinopines A or B of donor strains harbouring nopaline Ti plasmids promotes plasmid transfer whereas preculture of the same strains in the presence of nopaline has no such effect. Similarly, preculture in the presence of agrocinopines C or D promotes Ti-plasmid transfer from strains harbouring agropine Ti-plasmids.
Similar content being viewed by others
References
Albinger G, Beiderbeck R (1977) Übertragung der Fähigkeit zur Wurzelinduction von Agrobacterium rhizogenes auf A. tumefaciens. Phytopathol Z 90:306–310
Chilton M-D, Drummond HJ, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11:263–271
Cooper D, Firmin JL (1977) Synthesis of nopaline (N2−1,3 dicarboxypropyl) arginine and isonopaline. Org Prep Proced Int 9:99–101
Depicker A, Van Montagu M, Schell J (1978) Homologous DNA sequences in different Ti-plasmids are essential for oncogenicity. Nature (London) 275:250–253
Ellis JG, Kerr A, Van Montagu M, Schell J (1979a) Agrobacterium: genetic studies on agrocin 84 production and the biological control of crown gall. Physiol Plant Pathol 15:311–319
Ellis JG, Kerr A, Tempé J, Petit A (1979b) Arginine catabolism: a new function of both octopine and nopaline Ti-plasmids of Agrobacterium. Mol Gen Genet 173:263–269
Ellis JG, Murphy PJ (1981) Four new opines from crown gall tumours — their detection and properties. Mol Gen Genet 181:36–43
Firmin JL, Fenwick RG (1977) N2(1–3 dicarboxypropyl) ornithine in crown gall tumours. Phytochemistry 16:761–762
Firmin JL, Fenwick GR (1978) Agropine — a major new plasmiddetermined metabolite in crown gall tumours. Nature (London) 276:842–844
Goldmann A, Thomas DW, Morel G (1969) Sur la structure de la nopaline métabolite anormale de certaines tumeurs de crowngall. CR Acad Sci (Paris) 268:852–854
Guyon P, Chilton M-D, Petit A, Tempé J (1980) Agropine in “null type” crown gall tumors; evidence for the generality of the opine concept. Proc Natl Acad Sci USA 77:269302697
Holsters M, Silva B, Van Vliet F, Genetello C, De Block M, Dhaese P, Depicker A, Inzé D, Engler G, Villarroel R, Van Montagu M, Schell J (1980) The functional organization of the nopaline A. tumefaciens plasmid pTiC58. Plasmid 3:212–230
Hooykaas PJJ (1979) Ph. D. thesis, Rijksuniversiteit Leiden
Keane PJ, Kerr A, New PB (1970) Crown gall of stone fruit. II. Identification and nomenclature of Agrobacterium isolates. Austr J Biol Sci 23:585–595
Kemp JD (1977) A new amino-acid derivative present in crown gall tumor tissue. Biochem Biophys Res Commun 74:862–868
Kerr A (1969) Transfer of virulence between isolates of Agrobacterium. Nature (London) 223:1175–1176
Kerr A, Htay K (1974) Biological control of crown gall through bacteriocin production. Physiol Plant Pathol 4:37–44
Kerr A, Manigault P, Tempé J (1977) Transfer of virulence in vivo and in vitro in Abrobacterium. Nature (London) 265:560–561
Kerr A, Roberts WP (1976) Agrobacterium: Correlation between and transfer of pathogenicity, octopine and nopaline metabolism and bacteriocin 84 sensitivity. Physiol Plant Pathol 9:205–211
Klapwijk PM, Schilperoort RA (1979) Negative control of octopine degradation and transfer genes of octopine Ti-plasmids in Agrobacterium tumefaciens. J Bacteriol 139:424–431
Klapwijk PM, Scheuldermon T, Schilperoort RA (1978) Co-ordinated regulation of octopine degradation and conjugative transfer of Ti-plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons. J Bacteriol 136:775–785
Lemmers M, De Beuckeleer M, Holsters M, Zambryski P, Depicker A, Hernalsteens JP, Van Montagu M, Schell J (1980) Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline crown gall tumors. J Mol Biol 144:355–378
Lioret C (1957) Les acides aminés libres des tissus de crown gall cultivés in vitro. CR Acad Sci (Paris) 244:2171–2174
Ménagé A, Morel G (1964) Sur la présence d'octopine dans les tissus de crown gall. CR Acad Sci (Paris) 259:2795–4796
Ménagé A, Morel G (1965) Sur la présence d'un acide aminé nouveau dans le tissu de crown-gall. CR Soc Biol (Paris) 159:561–562
Moore L, Warren G, Strobel G (1979) Involvement of a plasmid in the hairy root disease of plants caused by Agrobacterium rhizogenes. Plasmid 2:617–626
Murphy PJ, Roberts WP (1979) A basic for agrocin 84 sensitivity in Agrobacterium. J Gen Microbiol 114:207–213
Petit A, Dessaux Y, Tempé J (1978a) The biological significance of opines. I. A study of opine catabolism by Agrobacterium tumefaciens. Proc. IVth Int Conf Plant Pathogenic Bacteria, Angers, I.N.R.A., 143–152
Petit A, Tempé J (1978) Isolation of Agrobacterium Ti-plasmid regulatory mutants. Mol Gen Genet 167:147–155
Petit A, Tempé J, Kerr A, Hosters M, Van Montagu M, Schell J (1978b) Substrate induction of conjugative activity of Agrobacterium tumefaciens. Nature (London) 271:570–572
Schell J, Van Montagu M, De Beuckeleer M, De Block M, Depicker A, De Wilde M, Engler G, Genetello C, Hernalsteens JP, Holsters M, Seurinck J, Silva B, Van Vliet F, Villarroel R (1979) Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid an the plant host. Proc R Soc Lond B 204:251–266
Tate ME, Ellis JG, Kerr A, Tempé J, Murray KE, Shaw KJ (1982) Agropine: a revised structure. Carbohydr Res (in press)
Tempé J, Estrade C, Petit A (1978) The biological significance of opines. II. The conjugative activity of the Ti-plasmids of Agrobacterium tumefaciens. Proc IVth Conf Plant Pathogenic Bacteria, Angers, I.N.R.A. 153–160
Tempé J, Guyon P, Petit A, Ellis JG, Tate ME, Kerr A (1980) Préparation et propriétés de nouveaux substrats cataboliques pour deux types de plasmides oncogènes d'Agrobacterium tumefaciens. CR Acad Sci Paris, Série D 290:1173–1176
Van Larebeke N, Engler G, Holsters M, Van den Elsacker S, Zaenen I, Schilperoort RA, Schell J (1974) Large plasmid in Agrobacterium tumefaciens essential for crown gall inducing ability. Nature (London) 252:169–170
Van Larebeke N, Genetello C, Schell J, Schilperoort RA, Hermans AK, Hernalsteens JP, Van Montagu M (1975) Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature (London) 255:742–743
Watson B, Currier TC, Gordon MP, Chilton M-D, Nester EW (1975) Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol 123:255–264
White FF, Nester EW (1980) Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes. J Bacteriol 141:1134–1141
Author information
Authors and Affiliations
Additional information
Communicated by J. Schell
Rights and permissions
About this article
Cite this article
Ellis, J.G., Kerr, A., Petit, A. et al. Conjugal transfer of nopaline and agropine Ti-plasmids —The role of agrocinopines. Mol Gen Genet 186, 269–274 (1982). https://doi.org/10.1007/BF00331861
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331861