Summary
The coding properties have been analyzed of in vitro constructed λ recombinant phases carrying E. coli DNA fragments from around the structural gene for translation initiation factor IF3 (infC). This study shows that infC is expressed independently of the promoter of the threonyl-tRNA synthetase (thrS), which is the gene immediately preceding infC. It also shows that the two genes following infC, namely pheS and pheT, which form the phenylalanyl-tRNA synthetase operon, are not expressed from infC's promoter. Thus the four characterized genes of that region that were previously thought to be transcribed in the same direction are now shown to be expressed in vivo as three separate transcription units.
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References
Bachmann B, Brooks Low K (1980) Linkage map of E. coli K12, Edition 6. Microbiol Rev 44:1–56
Borck K, Beggs JD, Brammar WJ, Hopkins AS, Murray NE (1976) The construction in vitro of transducing derivatives of phage lambda. Mol Gen Genet 146:199–207
Chamberlain JP (1979) Fluorographic detection of radioactivity in polyacrylamide gels with water soluble fluor, sodium salicylate. Anal Biochem 98:132–135
Comer MM, Böck A (1976) Genes for the α and β subunits of the phenylalanyl-transfer ribonucleic acid synthetase of E. coli. J Bacteriol 127:923–933
Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory, New York
Dennert G, Henning V (1968) Tyrosine incorporating amber suppressors in E. coli K12 J Mol Biol 33:322–329
Hennecke H, Böck A, Thamale J, Nass G (1977) Threonyl-transfer ribonucleic acid synthetase from E. coli: subunit structure and genetic analysis of the structural gene by means of a mutated enzyme and of a specialized transducing λ bacteriophage. J Bacteriol 131:943–950
Howe JG, Yanov J, Meyer L, Johnston K, Hershey JWB (1978) Determination of protein synthesis initiation factor levels in crude lysates of E. coli by a sensitive radioimmune assay. Arch Biochem Biophys 191:813–820
Johnston EJ, Cohen G, Saint Girons I (1977) Threonyl transfer ribonucleic acid synthetase and the regulation of the threonine operon in E. coli.J Bacteriol 129:66–70
Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680–685
Laskey RA, Mills AD (1975) Quantitative film detection of tritium and 14C in polyacrylamide gels by fluorography. Eur J Biochem 56:335–341
Lestienne P, Dondon J, Plumbridge JA, Howe JG, Mayaux JF, Springer M, Blanquet S, Hershey JWB, Grunberg-Manago M (1982) Expression of the gene for E. coli initiation factor IF3 in vivo and in vitro. Overproduction in recombinant plasmid bearing strains, Eur J Biochem 123:483–488
Manly K, Signer E, Radding C (1969) Non essential functions of bacteriophage λ. Virology 37:177–188
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York
Mizuuchi K, Fukasawa T (1969) Chromosome mobilization in recmerodiploids of E. coli K12 following infection with bacteriophage λ. Virology 39:467–481
Neidhardt FC, Bloch PL, Pedersen S, Reeh S (1977) Chemical measurement of steady-state levels of ten aminoacyl-transfer ribonucleic acid synthetases in E. coli. J Bacteriol 129:378–387
Plumbridge JA, Springer M (1980) Genes for the two subunits of phenylalanyl-tRNA synthetase of E. coli are transcribed from the same promoter. J Mol Biol 144:595–600
Plumbridge JA, Springer M, Graffe M, Goursot R, Grunberg-Manago M (1980) Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation. Gene 11:33–42
Sacerdot C, Fayat G, Dessen P, Springer M, Plumbridge JA, Grunberg-Manago M, Blanquet S (1982) Sequence of a 1.26 kb DNA fragment containing the structural gene for E. coli initiation factor IF3: presence of an AUU initiator codon. EMBOJ (in press)
Schrenk WJ, Weisberg RA (1975) A simple method for making transducing lines of coliphage λ. Mol Gen Genet 137:101–107
Springer M, Graffe M, Hennecke H (1977) Specialized transducing phage for initiation factor IF3 gene in E. coli. Proc Natl Acad Sci USA 74:3970–3974
Springer M, Graffer M, Grunberg-Manago M (1979) Genetic organization of the E. coli chromosome around the structural gene for initiation factor IF3 (infC). Mol Gen Genet 169:337–343
Yates JL, Arfsten AE, Nomura M (1980) In vitro expression of E. coli ribosomal protein genes: autogenous inhibition of translation. Proc Natl Acad Sci USA 77:1837–1841
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Communicated by A. Böck
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Springer, M., Plumbridge, J.A., Trudel, M. et al. Transcription units around the gene for E. coli translation initiation factor IF3 (infC). Mol Gen Genet 186, 247–252 (1982). https://doi.org/10.1007/BF00331857
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DOI: https://doi.org/10.1007/BF00331857