Summary
Nonsense suppression by supE44 has been examined in a collection of 14 T4 gene 22 and gene 23 UAG mutants, for which the precise gene location is known. In concordance with previous studies, UAG followed by a pyrimidine was inefficiently suppressed. However, among positions with similar 3′ nucleotides, there was considerable variation in suppression efficiency. The competition between supE44 and Release Factor 1 (RF1) was also investigated following the introduction of a multicopy RF1 plasmid. An inverse relationship between the efficiency of suppression and RF1 competition was observed.
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References
Bossi L (1983) Context effects: Translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message. J Mol Biol 164:73–87
Bossi L, Roth JR (1980) The influence of codon context on genetic code translation. Nature 286:123–127
Feinstein SI, Altman S (1978) Context effects on nonsense codon suppression in Escherichia coli. Genetics 88:201–219
Fluck MM, Salser W, Epstein RH (1977) The influence of reading context upon the suppression of nonsense codons. Mol Gen Genet 151:137–149
Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Miller JH, Albertini AM (1983) Effects of surrounding sequence on the suppression of nonsense codons. J Mol Biol 164:59–71
Murgola EJ, Pagel FT, Hijazi KA (1984) Codon context effects in missense suppression. J Mol Biol 175:19–27
Parker ML, Christensen AC, Boosman A, Stockard J, Young ET, Doermann AH (1984) Nucleotide sequence of bacteriophage T4 gene 23 and the amino acid sequence of its product. J Mol Biol 180:399–416
Salser W (1969) The influence of the reading context upon the suppression of nonsense codons. Mol Gen Genet 105:125–130
Stormo GD, Schneider TD, Gold L (1986) Quantitative analysis of the relationship between nucleotide sequence and functional activity. Nucleic Acids Res 14:6661–6679
Weiss RB, Murphy JP, Gallant JA (1984) Genetic screen for cloned release factor genes. J Bacteriol 158:362–364
Yarus M, Foley LS (1985) Sense codons are found in specific contexts. J Mol Biol 182:529–540
Yarus M, Thompson RC (1983) Precision of protein biosynthesis. In: Beckwith J, Davies J, Gallant J (eds) Gene function in prokaryotes. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 23–64
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Communicated by K. Isono
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Edelmann, P., Martin, R. & Gallant, J. Nonsense suppression context effects in Escherichia coli bacteriophage T4. Mol Gen Genet 207, 517–518 (1987). https://doi.org/10.1007/BF00331625
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DOI: https://doi.org/10.1007/BF00331625