Summary
A mutant of Streptomyces coelicolor A3(2) highly resistant to chloramphenicol was selected. It had amplified some chromosomal DNA fragments to a copy number of 20–50. Some of the amplified fragments were cloned and used as hybridisation probes to investigate the spontaneous chloramphenicol-sensitive mutants which occur at high frequency in this species and the closely related species Streptomyces lividans 66. These investigations demonstrated that chloramphenicol sensitivity in both species is associated with large deletions that are at least 40 kb in length.
This is a preview of subscription content, log in via an institution to check access.
References
Altenbuchner J, Cullum J (1984) DNA amplification and an unstable arginine gene in Streptomyces lividans 66. Mol Gen Genet 195:134–138
Altenbuchner J, Cullum J (1985) Structure of an amplifiable DNA sequence in Streptomyces lividans 66. Mol Gen Genet 201:192–197
Bibb MJ, Hopwood DA (1981) Genetic studies of the fertility plasmid SCP2 and its SCP2* variants in Streptomyces coelicolor A 3(2). J Gen Microbiol 126:427–442
Danilenko VN, Starodubtseva LI, Navashin SM (1986) Regulation and expression of kanamycin and chloramphenicol resistance determinants in Streptomyces lividans 66. In: Szabo G, Biro S, Goodfellow M (eds) Biological, biochemical and biomedical aspects of Actinomycetes. Akademaii Kiado, Budapest, pp 79–81
Fishman SE, Hershberger CL (1983) Amplified DNA in Streptomyces fradiae. J Bacteriol 155:459–466
Flett F, Platt J, Cullum J (1987) DNA rearrangements associated with instability of an arginine gene in Streptomyces coelicolor A 3(2). J Basic Microbiol 27:3–10
Freeman RF, Hopwood DA (1978) Unstable naturally occurring resistance to antibiotics in Streptomyces. J Gen Microbiol 106:377–381
Freeman RF, Bibb MJ, Hopwood DA (1977) Chloramphenicol acetyltransferase-independent chloramphenicol resistance in Streptomyces coelicolor A 3(2). J Gen Microbiol 98:453–465
Hintermann G, Crameri R, Vogtli M, Hutter R (1984) Streptomycin-sensitivity in Streptomyces glaucescens is due to deletions comprising the structural gene coding for a specific phosphotransferase. Mol Gen Genet 196:513–520
Ishihara H, Nakano MM, Ogawara H (1985) Cloning of a gene from Streptomyces species complementing argG mutations. J Antibiot (Tokyo) 38:787–794
Kirby R, Lewis E (1981) Unstable genetic elements affecting streptomycin resistance in the streptomycin-producing organisms Streptomyces griseus NCIB 8506 and Streptomyces bikiniensis ISP 5235. J Gen Microbiol 122:351–355
Ono H, Hintermann G, Crameri R, Wallis G, Hutter R (1982) Reiterated DNA sequences in a mutant strain of Streptomyces glaucescens and cloning of the sequence in Escherichia coli. Mol Gen Genet 186:106–110
Potekhin YaA, Danilenko VN (1985) Kanamycin resistance determinant in Streptomyces rimosus: capacity for amplification in chromosome and for reversible genetic instability. Mol Biol (Mosk) 19:805–817
Redshaw PA, McCann PA, Pentella MA, Pogell BM (1979) Simultaneous loss of multiple differentiated functions in aerial mycelium-negative isolates of Streptomyces. J Bacteriol 137:891–899
Schrempf H (1983) Deletion and amplification of DNA sequences in melanin-negative variants of Streptomyces reticuli. Mol Gen Genet 189:501–505
Sermonti G, Petris A, Micheli MR, Lanfaloni L (1978) Chloramphenicol resistance in Streptomyces coelicolor A 3(2): possible involvement of a transposable element. Mol Gen Genet 164:99–103
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Flett, F., Cullum, J. DNA deletions in spontaneous chloramphenicol-sensitive mutants of Streptomyces coelicolor A3(2) and Streptomyces lividans 66. Mol Gen Genet 207, 499–502 (1987). https://doi.org/10.1007/BF00331621
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331621