Summary
A DNA plasmid resembling 2 μm DNA of Saccharomyces cerevisiae, pSR1, isolated from a strain of Zygosaccharomyces rouxii, has a cis-acting region, Z, for plasmid stability. The Z region was delimited to a sequence of at most 383 bp in a small unique region of the plasmid. The Z region is high in A:T pairs and contains three different pairs of short (ca. 25 bp) inverted repeats with 65% to 79% homology and three copies of direct repeats of 24 to 27 bp in length with 67% to 72% homology, but does not encode a noteworthy open reading frame. It was suggested that the Z region interacts with the S product(s) encoded by the same plasmid and with a specific host factor, but not with the other stabilization factor encoded by the P locus on the sPR1 molecule.
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Communicated by C.P. Hollenberg
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Jearnpipatkul, A., Hutacharoen, R., Araki, H. et al. A cis-acting locus for the stable propagation of yeast plasmid pSR1. Mol Gen Genet 207, 355–360 (1987). https://doi.org/10.1007/BF00331601
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DOI: https://doi.org/10.1007/BF00331601