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Nucleotide sequence of cDNA clones encoding the complete “33 kDa” precursor protein associated with the photosynthetic oxygen-evolving complex from spinach

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Summary

Several cDNA clones encoding the “33 kDa” protein associated with the photosynthetic water oxidation activity of spinach were sequenced. A 1208 bp insert of one of the clones encodes the entire 331 amino acid residues of the precursor protein including 84 amino acids (8.5 kDa) of the amino-terminal transit peptide, 49 bp of the 5′ and 111 bp of the 3′ untranslated segment of the mRNA. The 3′ poly(A) tail starts 19 bp downstream from a putative polyadenylation signal, TATAAA. The hydrophilic mature protein consists of 247 amino acid residues corresponding to an Mr of 26.5 kDa, which is 6.5 kDa smaller than the value determined by SDS-polyacrylamide gel electrophoresis (33–34 kDa), and shows a certain degree of conservation with the putative Mn-complexing active sites of bacterial Mn-dependent superoxide dismutases. The anatomy of the unusually long transit sequence is discussed with regard to current concepts of protein import into and protein routein within the organelle.

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Authors and Affiliations

  1. Botanisches Institut der Ludwig-Maximilians-Universität, Menzinger Str. 67, D-8000, München 19, Federal Republic of Germany

    A. Tyagi, J. Hermans, J. Steppuhn & R. G. Herrmann

  2. Arrhenius Laboratory, University of Stockholm, S-10691, Stockholm, Sweden

    Ch. Jansson

  3. Institut für Biochemie, Technische Universität Berlin, Franklinstr. 29, D-1000, Berlin 10, Federal Republic of Germany

    F. Vater

Authors
  1. A. Tyagi
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  2. J. Hermans
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  3. J. Steppuhn
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  4. Ch. Jansson
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  5. F. Vater
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  6. R. G. Herrmann
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Communicated by C.P. Hollenberg

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Tyagi, A., Hermans, J., Steppuhn, J. et al. Nucleotide sequence of cDNA clones encoding the complete “33 kDa” precursor protein associated with the photosynthetic oxygen-evolving complex from spinach. Mol Gen Genet 207, 288–293 (1987). https://doi.org/10.1007/BF00331591

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  • DOI: https://doi.org/10.1007/BF00331591

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