Summary
An experimental system to study cell cycle specific gene expression in plant cells was developed using protoplasts from tobacco cells synchronized by aphidicolin treatment. Chimeric plasmids consisting either of the chloramphenicol acetyltransferase (CAT) gene downstream of the cauliflower mosaic virus (CaMV) 35 S promoter or the nopaline synthase (nos) promoter were introduced into synchronized protoplasts of four cell cycle stages by electroporation. In the case of the CaMV 35 S promoter cyclic oscillation of CAT activity was observed which paralleled the cell cycle of the recipient cells. The peak of CAT activity was found in the S phase, while no such cyclic change was observed in the case of the nos promoter. This system clearly shows that it is feasible to search for a cell cycle specific promoter. The significance of these observations is discussed in relation to the study of plant cells.
Similar content being viewed by others
References
Artishevsky A, Grafsky A, Lee AS (1985) Isolation of a mammalian sequence capable of conferring cell cycle to a heterologous gene. Science 230: 1061–1063
Cold Spring Harbor Laboratory (1985) Molecular biology of plants. A laboratory manual. Cold Spring Harbor Laboratory, New York, p 50
Fromm ME, Taylor LP, Walbot V (1985) Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci USA 82: 5824–5828
Gorman CM, Moffat LF, Howard BH (1982) Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol 2: 1044–1051
Guilley H, Dudley RK, Jonard G, Balazs E, Richards KE (1982) Transcription of cauliflower mosaic virus DNA: Detection of promoter sequences, and characterization of transcripts. Cell 30: 763–773
Hanly SM, Bleecker GC, Heintz N (1985) Identification of promoter elements necessary for transcriptional regulation of human histone H4 gene in vitro. Mol Cell Biol 5: 380–389
Heintz N, Roeder RG (1984) Transcription of human histone genes in extracts from synchronized HeLa cells. Proc Natl Acad Sci USA 81: 2713–2717
Heintz N, Sive HL, Roeder RG (1983) Regulation of human histone gene expressions: Kinetics of accumulation and changes in the rate of synthesis and in the half-lives of individual histone mRNAs during the HeLa cell cycle. Mol Cell Biol 3: 530–550
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 273–280
Nagata T, Okada K, Takebe I, Matsui C (1981) Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (liposomes). Mol Gen Genet 184: 161–165
Nagata T, Okada K, Takebe I (1982) Mitotic protoplasts and their infection with tobacco mosaic virus RNA encapsulated in liposomes. Plant Cell Rep 1: 250–252
Okada K, Nagata T, Takebe I (1986a) Introduction of functional RNA into plant protoplasts by electroporation. Plant Cell Physiol 27: 619–626
Okada K, Takebe I, Nagata T (1986b) Expression and integration of genes introduced into highly synchronized plant protoplasts. Mol Gen Genet 205: 398–403
Weber G, de Groot E, Schweiger H-G (1986) Synchronization of protoplasts of Glycine max (L.) Merr and Brassica napus (L.) Planta 168: 273–280
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Nagata, T., Okada, K., Kawazu, T. et al. Cauliflower mosaic virus 35 S promoter directs S phase specific expression in plant cells. Mol Gen Genet 207, 242–244 (1987). https://doi.org/10.1007/BF00331584
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331584