Summary
E. coli K12 transformants, selected as leu + or pyrA + transformants, were analysed for inheritance of some closely linked unselected markers. Based on the observation that the number of recombinants which require, besides an integration event, one or more crossing-over events was negligible, a simple mapping function was deduced. The function L=e −kd, which directly relates observed linkage of an unselected marker and the relative distance of that unselected marker to the selected marker, gave a consistent interpretation of the experimental results.
This is a preview of subscription content, log in via an institution to check access.
References
Cosloy, Sh.D., Oishi, M.: The nature of the transformation process in Escherichia coli K12. Molec. gen. Genet. 124, 1–10 (1973)
Darlington, A.J., Bodmer, W.F.: Events occuring at the site of integration of a DNA molecule in Bacillus subtilis transformation. Genetics 60, 681–684 (1968)
Ephrussi-Taylor, H., Gray, T.C.: Genetic studies of recombining DNA in pneumococcal transformation. J. gen. Physiol. 49 (6), 183–196 (1966)
McFall, E.: Mapping of the D-serine deaminase region in Escherichia coli K12. Genetics 55, 91–99 (1967)
Taylor, A.L., Trotter, C.D.: Revised linkage map of Escherichia coli. Bact. Rev. 31, 332–353 (1967)
Taylor, A.L., Trotter, C.D.: Linkage map of Escherichia coli strain K-12. Bact. Rev. 36, 504–524 (1972)
Wu, T.T.: A model for three-point analysis of random general transduction. Genetics 54, 405–410 (1966)
Author information
Authors and Affiliations
Additional information
Communicated by G. Bertani and F. Stahl
Rights and permissions
About this article
Cite this article
Hoekstra, W.P.M., de Haan, P.G., Bergmans, J.E.N. et al. Transformation in E. coli K12: Relation of linkage to distance between markers. Molec. gen. Genet. 145, 109–110 (1976). https://doi.org/10.1007/BF00331565
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331565