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Chromosomal mapping of tRNA genes from Dictyostelium discoideum

Molecular and General Genetics MGG volume 207, pages 176–187 (1987)Cite this article

Summary

Different wild-type isolates of Dictyostelium discoideum exhibit extensive polymorphism in the length of restriction fragments carrying tRNA genes. These size differences were used to study the organisation of two tRNA gene families which encode a tRNAVal(GUU) and a tRNAVal(GUA) gene. The method used involved a combination of classitics. The tRNA genes were mapped to specific linkage groups (chromosomes) by correlating the presence of polymorphic DNA bands that hybridized with the tRNA gene probes with the presence of genetic markers for those linkage groups. These analyses established that both of the tRNA gene families are dispersed among sites on several of the chromosomes. Information of nine tRNAVal(GUU) genes from the wild-type isolate NC4 was obtained: three map to linkage group I (C, E, F,), two map to linkage group II (D, I), one maps to linkage group IV (G), one, which corresponds to the cloned gene, maps to either linkage group III or VI (B), and two map to one of linkage groups III, VI or VIII (A, H). Six tRNAVal(GUA) genes from the NC4 isolate were mapped; one to linkage group I (D), two to linkage group III, VI or VII (B, C) and three to linkage group VII or III (A, E, F).

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Author information

Author notes

  1. Keith L. Williams

    Present address: School of Biological Sciences, Macquarie University, 2113, North Ryde, N.S.W., Australia

Affiliations

  1. Institut für Physiologische Chemie, Universität Erlangen-Nürnberg, Fahrstraße 17, D-8520, Erlangen, Federal Republic of Germany

    Theodor Dingermann & Elfriede Amon

  2. Max Planck Intitut für Biochemie, D-8033, Martinsried bei München, Federal Republic of Germany

    Keith L. Williams & Dennis L. Welker

  3. Department of Biology, Utah State University, 84322-5500, Logan, UT, USA

    Dennis L. Welker

Authors
  1. Theodor Dingermann
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  2. Elfriede Amon
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  3. Keith L. Williams
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  4. Dennis L. Welker
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Additional information

Communicated by A. Böck

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Dingermann, T., Amon, E., Williams, K.L. et al. Chromosomal mapping of tRNA genes from Dictyostelium discoideum . Mol Gen Genet 207, 176–187 (1987). https://doi.org/10.1007/BF00331507

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  • DOI: https://doi.org/10.1007/BF00331507

Key words

  • Dictyostelium discoideum
  • Restriction fragment length polymorphism
  • tRNA genes
  • Chromosomal organization
  • Milti-copy genes