Summary
Vervet monkeys were given horseradish peroxidase intravenously. Subsequent studies with the electron microscope showed:
-
1.
The peroxidase molecules penetrated the capillary walls of the ciliary processes and “filled” the stroma and the intercellular spaces as far as the basal junctional complex of the superficial epithelial cells.
-
2.
The ciliary channels filled rapidly with peroxidase molecules.
-
3.
No peroxidase penetrated beyond the basal junctional complex of the superficial cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bairati, A., Jr., Orzalesi, N.: The ultrastructure of the epithelium of the ciliary body. A study of the junctional complexes and of the changes associated with the production of plasmoid aqueous humour. Z. Zellforsch. 69, 635–658 (1966).
Bill, A.: The albumin exchange in the rabbit eye. Acta physiol. scand. 60, 18–29 (1964).
—: The protein exchange in the eye with aspects on conventional and uveo-scleral bulk drainage of aqueous humour in primates. In: The structure of the eye, p. 313–320 (J. W. Rohen, ed.). Stuttgart: F. K. Schattauer 1965.
Cotran, R. S., Karnovsky, M. J.: Vascular leakage induced by horseradish peroxidase in the rat. Proc. Soc. exp. Biol. (N. Y.) 126, 557–561 (1967).
Davson, H.: Physiology of the eye, 2nd ed. London: J. & A. Churchill Ltd. 1963.
Farquhar, M. G., Palade, G. E.: Junctional complexes in various epithelia. J. Cell Biol. 17, 375–412 (1963).
Graham, R. C., Karnovsky, M. J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966).
Karnovsky, M. J.: Vesicular transport of exogenous peroxidase across capillary endothelium into the T system of muscle. J. Cell Biol. 27, 49A (1965).
—: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J. Cell Biol. 35, 213–236 (1967).
Kinsey, V. E., Reddy, D. V. N.: Chemistry and dynamics of aqueous humour. In: The rabbit in eye research, p. 218–319 (J. H. Prince, ed.). Springfield: Charles C. Thomas Publisher 1964.
Klapper, M. H., Hackett, D. P.: Investigations of the multiple components of commercial horseradish peroxidase. Biochim. biophys. Acta (Amst.) 96, 272–282 (1956).
Smelser, G. K., Pei, Y. F.: Cytological basis of protein leakage into the eye following paracentesis. An electron microscopic study. Invest. Ophthal. 4, 249–263 (1965).
Vegge, T.: The structure of the ciliary processes in the vervet monkey (Cercopithecus aethiops). (In prep.)
- A study of the ultrastructure of the small iris vessels and their permeability to horseradish peroxidase in the vervet monkey (Cercopithecus aethiops). (In prep.)
- Winther, F. Ø., Olsen, B. R.: Horseradish peroxidase in plasma studied by gel filtration. (In prep.)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vegge, T. An epithelial blood-aqueous barrier to horseradish peroxidase in the ciliary processes of the vervet monkey (Cercopithecus aethiops). Z. Zellforsch. 114, 309–320 (1971). https://doi.org/10.1007/BF00331458
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331458