Skip to main content
SpringerLink
Log in

Recognition and gene expression during the incompatibility reaction in Petunia hybrida L.

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The influence of proteins and RNA from unpollinated, inter-and intraspecifically pollinated styles and from pollen on pollen tube growth in vivo was tested by their application to the stigma prior to pollination. No RNA sample nor proteins from unpollinated styles were effective, whereas pollen tubes were shortened if the S-genotypes of the pollen matched that of the style from which the proteins originated and lengthened if not so. Pollen exudate proteins seemed to have the reversed effect. Active proteins could also be obtained by injection of RNA from pollinated styles into egg cells of Xenopus. Fractionation by means of preparative electrophoresis and isoelectric focusing leaded to two groups of active polypeptides with molecular weights of about 11,000 and 6,000. Application of these polypeptides to the stigma changed the gene expression of the style. Based upon these data a model to explain the recognition part of the incompatibility reaction is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ascher, P. D.: A gene action model to explain gametophytic self-incompatibility. Euphytica 15, 179–183 (1966)

    Google Scholar 

  • Ascher, P. D.: The influence of RNA-synthesis inhibitors on in vivo pollen tube growth and the self-incompatibility reaction in Lilium longiflorum Thunb. Theoret. appl. Genet. 41, 75–78 (1971)

    Google Scholar 

  • Britten, R. J., Davidson, E. H.: Gene regulation in higher cells. Science 165, 349–357 (1969)

    Google Scholar 

  • Campbell, R. J., Ascher, P. D.: Reduced pollen tube lengths in Lilium longiflorum Thunb. styles injected at pollination with phenol-extracted nucleic acids. Incompatibility News-letter 4, 33–37 (1974)

    Google Scholar 

  • Davidson, E. H., Britten, R. J.: Note on the control of gene expression during development. J. theoret. Biol. 32, 123–130 (1971)

    Google Scholar 

  • Donk, J. A. W. M. van der: Differential synthesis of RNA in self- and cross-pollinated styles of Petunia hybrida L. Molec. gen. Genet. 131, 1–8 (1974a)

    Google Scholar 

  • Donk, J. A. W. M. van der: Synthesis of RNA and protein as a function of time and type of pollen tube-style interaction in Petunia hybrida L. Molec. gen. Genet. 134, 93–98 (1974b)

    Google Scholar 

  • Donk, J. A. W. M. van der: Translation of plant messenger in egg cells of Xenopus laevis. Nature (Lond.) 256, 674–675 (1975)

    Google Scholar 

  • Howlett, B. J., Knox, R. B., Paxton, J. D., Heslop-Harrison, J.: Pollen-wall proteins: physicochemical characterization and role in self-incompatibility in Cosmos bipinnatus. Proc. roy. Soc. B 188, 167–182 (1975)

    Google Scholar 

  • Jacob, F., Monod, J.: Genetic regulatory mechanisms in the synthesis of proteins. J. molec. Biol. 3, 318–356 (1961)

    Google Scholar 

  • Koch, H. J. A., Backx, J.: A miniature electrofocusing method. Science Tools 16, 44–47 (1969)

    Google Scholar 

  • Kroes, H. W.: An enzyme theory of self-incompatibility. Incompatibility Newsletter 2, 5–14 (1973)

    Google Scholar 

  • Lewis, D.: A protein dimer hypothesis on incompatibility. In: Genetics to-day (S. J. Geerts, ed.). vol. 3, p. 657–663. London Pergamon Press 1965

    Google Scholar 

  • Linskens, H. F.: Biochemistry of incompatibility. In: Genetics to-day (S. J. Geerts, ed.), vol. 3, p. 629–635. London: Pergamon Press 1965

    Google Scholar 

  • Linskens, H. F.: The physiological basis of incompatibility in angiosperms. Biol. J. Linnean Soc. 7, Suppl. 1, 143–152 (1975)

    Google Scholar 

  • Linskens, H. F., Esser, K.: Über eine spezifische Anfärbung der Pollenschläuche im Griffel und die Zahl der Kallosepropfen nach Selbstung und Fremdung. Naturwissenschaften 44, 10 (1957)

    Google Scholar 

  • Linskens, H. F., Havez, R., Linder, R., Salden, M., Randoux, A., Laniez, D., Coustaut, D.: Etude des glycanne-hydrolases au cours de la croissance du pollen chez Petunia hybrida auto-incompatible. Bull. Soc. Pharm. France du Nord 1, 1–16 (1970)

    Google Scholar 

  • Linskens, H. F., Spanjers, A. W.: Changes of the electrical potential in the transmitting tissue of Petunia styles after cross- and self-pollination. Incompatibility Newsletter 3, 8–85 (1973).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, Section Molecular Developmental Biology, University of Nijmegen, Toernooiveld, Nijmegen, The Netherlands

    J. A. W. M. van der Donk

Authors
  1. J. A. W. M. van der Donk
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. Melchers

Rights and permissions

Reprints and permissions

About this article

Cite this article

van der Donk, J.A.W.M. Recognition and gene expression during the incompatibility reaction in Petunia hybrida L.. Molec. gen. Genet. 141, 305–316 (1975). https://doi.org/10.1007/BF00331452

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00331452

Keywords

Search

Navigation