Summary
The fine structure of ovarian steroid-producing cells of the domestic fowl as seen after administration of steroids is described. Diaethylstilboestrol, estradiol and hydroxyprogesterone were given as intramuscular injections for a 28-days period. The main cytoplasmic changes of the steroid-producing cells were an increase in the number and the size of lipid droplets due to a possible expansion of the endoplasmic reticulum and a confluence of the smooth endoplasmic reticulum and the lipid droplets. The mitochondria became paler, markedly reduced in size and number, and contained less cristae. The Golgi apparatus became smaller and tended to disappear. The most prominent nuclear changes were irregular outline of the nuclear membrane, shrinkage of the nucleus, formation of pseudo-inclusions, decreased number of nuclear bodies with a tendency to vacuolization and small nucleoli. These alterations have never been demonstrated in ovarian steroid-producing cells before, and they are consistent with atrophic changes observed in steroid-producing cells in other organs. The present study substantiates the view that the thecal gland must be concerned with endocrine function. The possible functions of the different cell types of the thecal gland are discussed.
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Adams, J. L.: Progesterone-induced unseasonable molt in single comb White Leghorn pullets. Poult. Sci. 34, 702–707 (1955).
—: A comparison of different methods of progesterone administration to the fowl in affecting egg production and molt. Poult. Sci. 35, 323–326 (1956).
Appelgren, L.-E.: Sites of steroid hormone formation. Autoradiographic studies using labelled precursors. Acta physiol. scand., Suppl. 301, 1–108 (1967).
Bjersing, L.: On the morphology and endocrine function of granulosa cells in ovarian follicles and corpora lutea. Acta endocr. (Kbh.), Suppl. 125, 1–23 (1967).
—: On the ultrastructure of granulosa lutein cells in porcine corpus luteum. With special reference to endoplasmic reticulum and steroid hormone synthesis. Z. Zellforsch. 82, 187–211 (1967).
Breneman, W. R.: Reproduction in birds: the female. In: comparative physiology of reproduction. Mem. Soc. Endocr. 4, 94–110 (1955).
—: Steroid hormones and the development of the reproductive system in the pullet. Endocrinology 58, 262–271 (1956).
Bullough, W. S.: The effects of oestrone on the ovary of the mouse. J. Endocr. 3, 235–243 (1943).
Dahl, E.: Studies of the fine structure of ovarian interstitial tissue. 1. A comparative study of the fine structure of the ovarian interstitial tissue in the rat and the domestic fowl. J. Anat. (Lond.) (1970) (accepted for publication).
—: Studies of the fine structure of ovarian interstitial tissue. 2. The ultrastructure of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 195–211 (1970).
—: Studies of the fine structure of ovarian interstitial tissue. 3. The innervation of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 212–226 (1970).
- Studies of the fine structure of ovarian interstitial tissue. 5. Effects of gonadotropins on the thecal gland of the domestic fowl. Z. Zellforsch. (1970) (accepted for publication).
—: Studies of the fine structure of ovarian interstitial tissue. 6. Effects of clomiphene on the thecal gland of the domestic fowl. Z. Zellforsch. 109, 227–244 (1970).
—: The fine structure of nuclear inclusions. J. Anat. (Lond.) 106, 255–262 (1970).
Davies, J., Broadus, C. D.: Studies on the fine structure of ovarian steroid-secreting cells in the rabbit. I. The normal interstitial cells. Amer. J. Anat. 128, 441–474 (1968).
Enders, A. C., Lyons, W. R.: Observations on the fine structure of lutein cells. II. The effects of hypophysectomy and mammotrophic hormone in the rat. J. Cell Biol. 22, 127–141 (1964).
Everett, J. W.: The mammalian female reproductive cycle and its controlling mechanisms. In: Sex and internal secretions, 3rd ed., vol. I, (W. C. Young ed.), p. 497–555. Baltimore: Williams & Wilkins Co. 1961.
Falck, B.: Site of production of oestrogen in rat ovary as studied in micro-transplants. Acta physiol. scand. 47, 1–101 (1959).
Fraps, R. M.: The varying effects of sex hormones in birds. In: Comparative physiology of reproduction. Mem. Soc. Endocr. 4, 205–219 (1955).
—, Hooker, C. W., Forbes, T. R.: Progesterone in blood plasma of the ovulating hen. Science 108, 86–87 (1948).
—: Progesterone in blood plasma of cocks and nonovulating hens. Science 109, 493 (1949).
Himeno, K., Tanabe, Y.: Mechanism of molting in the hen. Poult. Sci. 36, 835–842 (1957).
Ichii, S., Kobayashi, S., Yago, N., Omata, S.: Role of plasma cholesterol in rat adrenal corticosteroidogenesis in vitro. Endocr. jap. 14, 138–142 (1957).
Idelman, S.: Contribution a la cytophysiolgic infrastructurale de la corticosurrénale chez le Rat albinos. Ann. Sci. nat. Zool. 8, 205–362 (1966).
Kjaerheim, Å.: Studies of adrenocortical ultrastructure. 2. The interrenal cell of the domestic fowl as seen after glutaraldehyde perfusion fixation. Z. Zellforsch. 91, 429–455 (1968a).
—: Studies of adrenocortical ultrastructure. 3. Effects of dexamethasone and medroxy-progesterone on interrenal cells of the domestic fowl. Z. Zellforsch. 91, 456–474 (1968b).
—: Studies of adrenocortical ultrastructure. 4. Effects of ACTH on interrenal cells of the domestic fowl. J. Microscopie 7, 715–738 (1968c).
—: Studies of adrenocortical ultrastructure. 1. Aldehyde perfusion fixation of the domestic fowl. Acta anat. (Basel) 74, 424–453 (1969).
Layne, D. S., Common, R. H., Maw, W. A., Fraps, R. M.: Presence of progesterone in extracts of ovaries of laying hens. Proc. Soc. exp. Biol. (N.Y.) 94, 528–529 (1957).
Marlow, H. W., Richert, D.: Estrogen of the fowl. Endocrinology 26, 531–534 (1940).
Marshall, A. J., Coombs, C. J. F.: The interaction of environmental, internal and behavioural factors in the rook, Corvus frugilegus Linnaeus. Proc. Zool. Soc. Lond. 128, 545–589 (1957).
Morrow, L. B., Burrow, G. N., Mulrow, P. T.: Inhibition of adrenal protein synthesis by steroids in vitro. Endocrinology 80, 883–888 (1967).
O'Grady, J. E.: Oestrogens in plasma from the domestic fowl. In: Physiology of the domestic fowl. (C. Horton-Smith and E.C. Amoroso, eds.), p. 23–29. Edinburgh and London: Oliver & Boyd 1966.
Penney, D. P., Barrnett, R. J.: The fine structural localization and selective inhibition of nucleosidephosphatases in the rat adrenal cortex. Anat. Rec. 70, 543–556 (1938).
Phillips, R. E.: Ovarian response of hens and pullets to injections of ambinon. Poult. Sci. 22, 368–373 (1943).
—: Endocrine mechanisms of the failure of Pintails (Anas acuta) to reproduce in captivity. Diss. Abstr. 20, 3033 (1960).
Rennels, E. G.: Influence of hormones on the histochemistry of ovarian interstitial tissue in the immature rat. Amer. J. Anat. 88, 63–108 (1951).
—: Observations on the ultrastructure of luteal cells from PMS and PMS-HCG treated immature rats. Endocrinology 79, 373–386 (1966).
Reynolds, B. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Rothchild, I., Fraps, R. M.: The interval between normal release of ovulating hormone and ovulation in the domestic hen. Endocrinology 44, 134–140 (1949a).
—: The induction of ovulating hormone release from the pituitary of the domestic hen by means of progesterone. Endocrinology 44, 141–149 (1949b).
Ryter, A., Kellenberger, E.: L'inclusion au polyester pour l'ultramicrotomie. J. Ultrastruct. Res. 2, 200–214 (1958).
Siekevitz, P.: Protoplasma: endoplasmic reticulum and microsomes and their properties. Ann. Rev. Physiol. 25, 15–40 (1963).
Szabo, D., Stark, E., Varga, B.: The localisation of acid phosphatase activity changes in lysosomes in the adrenal zona fasciculata of intact and hypophysectomized rats following ACTH administration. Histochemie 10, 321–328 (1967).
Taber, E.: Androgen secretion in the fowl. Endocrinology 48, 6–16 (1951).
Tienhoven, A. van: Endocrinology of reproduction in birds. In: Sex and internal secretions, 3rd ed., vol. II, (W. C. Young ed.), p. 1088–1169. Baltimore: Williams & Wilkins Co. 1961.
Williams, P. C.: Effect of stilboestrol on the ovaries of hypophysectomized rats. Nature (Lond.) 145, 388–389 (1940).
Witschi, E., Fugo, N. W.: Response of sex characters of the adult female starling to synthetic hormones. Proc. Soc. exp. Biol., (N.Y.) 45, 10–14 (1940).
Young, W. C.: The mammalian ovary. In: Sex and internal secretions (W. C. Young, ed.), 3rd ed., vol. I, p. 449–496. Baltimore: Williams & Wilkins Co. 1961.
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Dahl, E. Studies of the fine structure of ovarian interstitial tissue. Z. Zellforsch. 113, 111–132 (1971). https://doi.org/10.1007/BF00331205
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DOI: https://doi.org/10.1007/BF00331205