Summary
Induction of the SOS response in Escherichia coli results in an increase in the relative rate of synthesis of single-stranded DNA binding protein (SSB). In contrast to RecA protein, this increase is slow and does not lead to higher SSB levels. The significance of ssb induction to SOS repair is discussed.
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References
Alazard RJ (1983) Study of the expression of UVRA and SSB proteins in vivo in lambda phages containing the uvrA and ssb genes of Escherichia coli. Mutat Res 109:155–168
Baluch J, Chase JW, Sussman R (1980) Synthesis of recA protein and induction of bacteriophage lambda in single-strand deoxyribonucleic acid-binding protein mutants of Escherichia coli. J Bacteriol 144:489–498
Bobst EV, Bobst AM, Perrino FW, Meyer RR, Rein DC (1985) Variability in the nucleic acid binding site size and the amount of single-stranded DNA-binding protein in Escherichia coli. FEBS Lett 181:133–137
Brandsma JA, Bosch D, Backendorf C, van de Putte P (1983) A common regulatory region shared by divergently transcribed genes of the Escherichia coli SOS system. Nature 305:243–245
Brandsma JA, Bosch D, de Ruyter M, van de Putte P (1985) Analysis of the regulatory region of the ssb gene of Escherichia coli. Nucleic Acids Res 13:5095–5109
Glassberg J, Meyer RR, Kornberg A (1979) Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J Bacteriol 140:14–19
Golub EI, Low KB (1983) Indirect stimulation of genetic recombination. Proc Natl Acad Sci USA 80:1401–1405
Johnson BF (1977) Genetic mapping of the lexC-113 mutation. Mol Gen Genet 157:91–97
Kenyon CJ, Walker GC (1980) DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli. Proc Natl Acad Sci USA 77:2819–2823
Lieberman HB, Witkin EM (1981) Variable expression of the ssb-1 allele in different strains of Escherichia coli K12 and B: differential suppression of its effects on DNA replication, DNA repair and ultraviolet mutagenesis. Mol Gen Genet 183:348–355
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Meyer RR, Glassberg J, Kornberg A (1979) An Escherichia coli mutant defective in single-strand binding-protein is defective in DNA replication. Proc Natl Acad Sci USA 76:1702–1705
Meyer RR, Glassberg J, Scott JV, Kornberg A (1980) A temperature-sensitive single-stranded DNA-binding protein from Escherichia coli. J Biol Chem 255:2897–2901
Meyer RR, Rein DC, Glassberg J (1982a) The product of the lexC gene of Escherichia coli is single-stranded DNA-binding protein. J Bacteriol 150:433–435
Meyer RR, Voegele DW, Ruben SM, Rein DC, Trela JM (1982b) Influence of single-stranded DNA-binding protein on recA induction in Escherichia coli. Mutat Res 94:299–313
Rebollo JE, Moreau PL, Blanco M, Devoret R (1984) Restoration of RecA protein activity by genetic complementation. Mol Gen Genet 195:83–89
Salles B, Paoletti C, Villani G (1983) Lack of single-strand DNA-binding protein amplification under conditions of SOS induction in E. coli. Mol Gen Genet 189:175–177
Sancar A, Williams KR, Chase JW, Rupp WD (1981) Sequences of the ssb gene and protein. Proc Natl Acad Sci USA 78:4274–4278
Villani G, Pierre A, Salles P (1984) Quantification of SSB protein in E. coli and its variation during RecA protein induction. Biochemie 66:471–476
Walker GC (1985) Inducible DNA repair systems. Annu Rev Biochem 54:425–457
Whittier RC, Chase JW (1981) DNA repair in E. coli strains deficient in single-strand DNA binding protein. Mol Gen Genet 183:341–347
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Communicated by R. Devoret
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Perrino, F.W., Rein, D.C., Bobst, A.M. et al. The relative rate of synthesis and levels of single-stranded DNA binding protein during induction of SOS repair in Escherichia coli . Mol Gen Genet 209, 612–614 (1987). https://doi.org/10.1007/BF00331171
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DOI: https://doi.org/10.1007/BF00331171