Summary
A novel plasmid vector, pAMH70 carrying both the lamB and nusA genes of Escherichia coli K12 was constructed. Introduction of this plasmid into Salmonella typhimurium LT2 renders this bacterium both sensitive to λ adsorption and able to sustain growth and lysogenization by λ. Using this strain as a recipient, stable gene fusions to the gene encoding a major outer membrane porin protein OmpC, were constructed with a λ vehicle λ placMu. To confirm the actual site of fusions they were genetically mapped and transducing phages carrying the ompC-lacZ fusion were isolated and relysogenized. The fusions were also shown to be to ompC by their regulatory properties.
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Harkki, A., Karkku, H. & Tapio Palva, E. Use of λ vehicles to isolate ompC-lacZ gene fusions in Salmonella typhimurium LT2. Mol Gen Genet 209, 607–611 (1987). https://doi.org/10.1007/BF00331170
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DOI: https://doi.org/10.1007/BF00331170